ArticleEukaryotic cell2002
Role of histone deacetylation in developmentally programmed DNA rearrangements in Tetrahymena thermophila.
Article in Eukaryotic cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Programmed DNA Elimination in Vertebrates.Annual review of animal biosciences · 2021Review
- Exploring the Histone Acetylation Cycle in the Protozoan ModelFrontiers in cell and developmental biology · 2020Review
- RNAi-dependentGenes & development · 2019Article
- A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.Nucleic acids research · 2017Article
- SUMOylation is developmentally regulated and required for cell pairing during conjugation in Tetrahymena thermophila.Eukaryotic cell · 2015Article
- Sirtuin-mediated nuclear differentiation and programmed degradation in Tetrahymena.BMC cell biology · 2011Article
- Dynamic nuclear reorganization during genome remodeling of Tetrahymena.Biochimica et biophysica acta · 2008Review
- Spatial and temporal plasticity of chromatin during programmed DNA-reorganization in Stylonychia macronuclear development.Epigenetics & chromatin · 2008Article
- Study of an RNA helicase implicates small RNA-noncoding RNA interactions in programmed DNA elimination in Tetrahymena.Genes & development · 2008Article
- A class II histone deacetylase acts on newly synthesized histones in Tetrahymena.Eukaryotic cell · 2008Article
- RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena.Genes & development · 2007Article
- snRNA and heterochromatin formation are involved in DNA excision during macronuclear development in stichotrichous ciliates.Eukaryotic cell · 2005Article
- Communication between parental and developing genomes during tetrahymena nuclear differentiation is likely mediated by homologous RNAs.Genetics · 2005Article
- Role of micronucleus-limited DNA in programmed deletion of mse2.9 during macronuclear development of Tetrahymena thermophila.Eukaryotic cell · 2004Article
- Histone H3 lysine 9 methylation is required for DNA elimination in developing macronuclei in Tetrahymena.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Developmentally regulated chromosome fragmentation linked to imprecise elimination of repeated sequences in paramecia.Eukaryotic cell · 2003Article
- Diverse sequences within Tlr elements target programmed DNA elimination in Tetrahymena thermophila.Eukaryotic cell · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In Tetrahymena, as in other ciliates, development of the somatic macronucleus during conjugation involves extensive and reproducible rearrangements of the germ line genome, including chromosome fragmentation and excision of internal eliminated sequences (IESs). The molecular mechanisms controlling these events are poorly understood. To investigate the role that histone acetylation may play in the regulation of these processes, we treated Tetrahymena cells during conjugation with the histone deacetylase inhibitor trichostatin A (TSA). We show that TSA treatment induces developmental arrests in the early stages of conjugation but does not significantly affect the progression of conjugation once the mitotic divisions of the zygotic nucleus have occurred. Progeny produced from TSA-treated cells were examined for effects on IES excision and chromosome breakage. We found that TSA treatment caused partial inhibition of excision of five out of the six IESs analyzed but did not affect chromosome breakage at four different sites. TSA treatment greatly delayed in some cells and inhibited in most the excision events in the developing macronucleus. It also led to loss of the specialized subnuclear localization of the chromodomain protein Pdd1p that is normally associated with DNA elimination. We propose a model in which underacetylated nucleosomes mark germ line-limited sequences for excision.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.