ArticleEukaryotic cell2002
The two nuclei of Giardia each have complete copies of the genome and are partitioned equationally at cytokinesis.
Article in Eukaryotic cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 103 citations in OpenAlex.
- Review
- Genetic Diversity of the Flavohemoprotein Gene ofInfection and drug resistance · 2020Article
- Recent advances in functional research in Giardia intestinalis.Advances in parasitology · 2020Review
- Identification of a TRBD zinc finger-interacting protein in Giardia duodenalis and its regulation of telomerase.Parasites & vectors · 2019Article
- Role of gamma-giardin in ventral disc formation of Giardia lamblia.Parasites & vectors · 2019Article
- Myosin-independent cytokinesis inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Constitutive aneuploidy and genomic instability in the single-celled eukaryote Giardia intestinalis.MicrobiologyOpen · 2016Article
- Binucleation to breed new plant species adaptable to their environments.Plant signaling & behavior · 2015Article
- Nuclear inheritance and genetic exchange without meiosis in the binucleate parasite Giardia intestinalis.Journal of cell science · 2012Article
- Visualization of chromosomes in the binucleate intestinal parasite Giardia lamblia.Parasitology research · 2011Article
- Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai isolates: evidence of genetic exchange or mixed infections?BMC microbiology · 2011Article
- Lipid metabolism in Giardia: a post-genomic perspective.Parasitology · 2011Review
- Transcriptome analyses of the Giardia lamblia life cycle.Molecular and biochemical parasitology · 2010Article
- Chromosome sequence maps of the Giardia lamblia assemblage A isolate WB.Trends in parasitology · 2010Review
- Behind the smile: cell biology and disease mechanisms of Giardia species.Nature reviews. Microbiology · 2010Review
- Large genomic differences between the morphologically indistinguishable diplomonads Spironucleus barkhanus and Spironucleus salmonicida.BMC genomics · 2010Article
- Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia.Parasitology research · 2010Article
- Rapid Diagnosis of Intestinal Parasitic Protozoa, with a Focus on Entamoeba histolytica.Interdisciplinary perspectives on infectious diseases · 2009Article
- Sequence map of the 3-Mb Giardia duodenalis assemblage A chromosome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Interaction of BOP1, a protein for ribosome biogenesis, with EB1 in Giardia lamblia.Parasitology research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Giardia lamblia is medically important as a cause of diarrhea and malabsorption throughout the world and is thought to be one of the earliest-branching eukaryotes on a phylogenetic tree. Nevertheless, the mechanisms of inheritance are largely unknown. The trophozoites of Giardia and other diplomonads are interesting in their possession of two nuclei that are identical or similar in several respects. They replicate at nearly the same time, have similar quantities of DNA, and are both transcriptionally active. We used fluorescence in situ hybridization to demonstrate that genes from each of the five chromosomes are found in both nuclei, confirming that each nucleus has at least one complete copy of the genome. This raises a second question. The alleles of a gene in different nuclei are expected to accumulate different mutations, but surprisingly, the degree of heterozygosity in a clone is very low. One possible mechanism for eliminating sequence differences between nuclei is that each daughter cell receives two copies of the same nucleus at cell division. We used trophozoites with a plasmid transfected into a single nucleus to demonstrate that the two nuclei are partitioned equationally at cytokinesis. The mechanism(s) by which homozygosity is maintained will require further investigation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.