ArticleEukaryotic cell2002
Amino acid-dependent Gcn4p stability regulation occurs exclusively in the yeast nucleus.
Article in Eukaryotic cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Regulation of Sensing, Transportation, and Catabolism of Nitrogen Sources in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2018Review
- Identification of Genes inG3 (Bethesda, Md.) · 2017Article
- Chromatin Association of Gcn4 Is Limited by Post-translational Modifications Triggered by its DNA-Binding in Saccharomyces cerevisiae.Genetics · 2016Article
- Accumulation of a threonine biosynthetic intermediate attenuates general amino acid control by accelerating degradation of Gcn4 via Pho85 and Cdk8.PLoS genetics · 2014Article
- Sumoylation of transcription factor Gcn4 facilitates its Srb10-mediated clearance from promoters in yeast.Genes & development · 2012Article
- Cdk1-dependent destruction of Eco1 prevents cohesion establishment after S phase.Molecular cell · 2011Article
- Degradation of Saccharomyces cerevisiae transcription factor Gcn4 requires a C-terminal nuclear localization signal in the cyclin Pcl5.Eukaryotic cell · 2009Article
- Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4.Molecular and cellular biology · 2008Article
- Specific amino acid residues in the basic helix-loop-helix domain of SRC-3 are essential for its nuclear localization and proteasome-dependent turnover.Molecular and cellular biology · 2007Article
- SCFCdc4-mediated degradation of the Hac1p transcription factor regulates the unfolded protein response in Saccharomyces cerevisiae.Molecular biology of the cell · 2007Article
- Yeast Gcn4p stabilization is initiated by the dissociation of the nuclear Pho85p/Pcl5p complex.Molecular biology of the cell · 2006Article
- Activator Gcn4p and Cyc8p/Tup1p are interdependent for promoter occupancy at ARG1 in vivo.Molecular and cellular biology · 2005Article
- Degradation of Hof1 by SCF(Grr1) is important for actomyosin contraction during cytokinesis in yeast.The EMBO journal · 2005Article
- Nuclear import of yeast Gcn4p requires karyopherins Srp1p and Kap95p.Molecular genetics and genomics : MGG · 2004Article
- Controlling transcription by destruction: the regulation of yeast Gcn4p stability.Current genetics · 2003Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The c-Jun-like transcriptional activator Gcn4p controls biosynthesis of translational precursors in the yeast Saccharomyces cerevisiae. Protein stability is dependent on amino acid limitation and cis signals within Gcn4p which are recognized by cyclin-dependent protein kinases, including Pho85p. The Gcn4p population within unstarved yeast consists of a small relatively stable cytoplasmic fraction and a larger less stable nuclear fraction. Gcn4p contains two nuclear localization signals (NLS) which function independently of the presence or absence of amino acids. Expression of NLS-truncated Gcn4p results in an increased cytoplasmic fraction and an overall stabilization of the protein. The same effect is achieved for the entire Gcn4p in a yrb1 yeast mutant strain impaired in the nuclear import machinery. In the presence of amino acids, controlled destabilization of Gcn4p is triggered by the phosphorylation activity of Pho85p. A pho85delta mutation stabilizes Gcn4p without affecting nuclear import. Pho85p is localized within the nucleus in the presence or absence of amino acids. Therefore, there is a strict spatial separation of protein synthesis and degradation of Gcn4p in yeast. Control of protein stabilization which antagonizes Gcn4p function is restricted to the nucleus.
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