ArticleJournal of clinical microbiology2002
Identification of Listeria species by microarray-based assay.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
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- Molecular tools for the selective detection of nine diatom species biomarkers of various water quality levels.International journal of environmental research and public health · 2015Article
- An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food.Frontiers in microbiology · 2015Review
- Genomic characterization of novel Listeria monocytogenes serotype 4b variant strains.PloS one · 2014Article
- Rapid and sensitive detection of Listeria ivanovii by loop-mediated isothermal amplification of the smcL gene.PloS one · 2014Article
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- Rapid identification of allergenic and pathogenic molds in environmental air by an oligonucleotide array.BMC infectious diseases · 2011Article
- Detection of representative enteropathogenic bacteria, Vibrio spp., pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, using a virulence factor gene-based oligonucleotide microarray.Journal of microbiology (Seoul, Korea) · 2010Article
- Probing the pan-genome of Listeria monocytogenes: new insights into intraspecific niche expansion and genomic diversification.BMC genomics · 2010Article
- Early identification of sepsis.Current infectious disease reports · 2010Article
- Oligonucleotide microarray for the identification of potential mycotoxigenic fungi.BMC microbiology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a rapid microarray-based assay for the reliable detection and discrimination of six species of the Listeria genus: L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L. seeligeri, and L. grayi. The approach used in this study involves one-tube multiplex PCR amplification of six target bacterial virulence factor genes (iap, hly, inlB, plcA, plcB, and clpE), synthesis of fluorescently labeled single-stranded DNA, and hybridization to the multiple individual oligonucleotide probes specific for each Listeria species and immobilized on a glass surface. Results of the microarray analysis of 53 reference and clinical isolates of Listeria spp. demonstrated that this method allowed unambiguous identification of all six Listeria species based on sequence differences in the iap gene. Another virulence factor gene, hly, was used for detection and genotyping all L. monocytogenes, all L. ivanovii, and 8 of 11 L. seeligeri isolates. Other members of the genus Listeria and three L. seeligeri isolates did not contain the hly gene. There was complete agreement between the results of genotyping based on the hly and iap gene sequences. All L. monocytogenes isolates were found to be positive for the inlB, plcA, plcB, and clpE virulence genes specific only to this species. Our data on Listeria species analysis demonstrated that this microarray technique is a simple, rapid, and robust genotyping method that is also a potentially valuable tool for identification and characterization of bacterial pathogens in general.
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