ArticleJournal of clinical microbiology2002
Development of a real-time fluorescence PCR assay for rapid detection of the diphtheria toxin gene.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Emerging Corynebacterium diphtheriae ST-212 cluster with truncated tox gene linked by possible sexual transmission.Infection · 2026Article
- Re-emergence of Diphtheria in Guinea: An Outbreak Analysis of Vaccination and Disease Control Perspectives.Open forum infectious diseases · 2025Article
- Challenges of Diphtheria Toxin Detection.Toxins · 2024Review
- A case of fulminant respiratory diphtheria in a 24-year-old Afghan refugee in Austria in May 2022: a case report.Infection · 2023Article
- Analysis ofPeerJ · 2023Article
- Respiratory Illness Caused by Corynebacterium diphtheriae and C. ulcerans, and Use of Diphtheria Antitoxin in the United States, 1996-2018.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Investigation of a Large Diphtheria Outbreak and Cocirculation of Corynebacterium pseudodiphtheriticum Among Forcibly Displaced Myanmar Nationals, 2017-2019.The Journal of infectious diseases · 2021Article
- Assessing the Genetic Diversity of Austrian Corynebacterium diphtheriae Clinical Isolates, 2011 to 2019.Journal of clinical microbiology · 2021Article
- Detection of toxin-producingIranian journal of microbiology · 2020Article
- Detection and Characterization of Diphtheria Toxin Gene-BearingJournal of clinical microbiology · 2020Article
- Draft Genome Sequences of Six Corynebacterium ulcerans Strains Isolated from Humans and Animals in Austria, 2013 to 2019.Microbiology resource announcements · 2020Article
- Molecular Characterization of Corynebacterium diphtheriae Outbreak Isolates, South Africa, March-June 2015.Emerging infectious diseases · 2017Article
- An isolated outbreak of diphtheria in South Africa, 2015.Epidemiology and infection · 2017Article
- Corynebacterium amycolatum: An Unexpected Pathogen in the Ear.Journal of clinical and diagnostic research : JCDR · 2015Article
- Pilus gene pool variation and the virulence of Corynebacterium diphtheriae clinical isolates during infection of a nematode.Journal of bacteriology · 2013Article
- Multiplex polymerase chain reaction to identify and determine the toxigenicity of Corynebacterium spp with zoonotic potential and an overview of human and animal infections.Memorias do Instituto Oswaldo Cruz · 2013Article
- Rapid detection and molecular differentiation of toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans strains by LightCycler PCR.Journal of clinical microbiology · 2011Article
- Multilocus sequence typing identifies evidence for recombination and two distinct lineages of Corynebacterium diphtheriae.Journal of clinical microbiology · 2010Article
- Novel Corynebacterium diphtheriae in domestic cats.Emerging infectious diseases · 2010Article
- International external quality assurance for laboratory diagnosis of diphtheria.Journal of clinical microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed and evaluated a real-time fluorescence PCR assay for detecting the A and B subunits of diphtheria toxin (tox) gene. When 23 toxigenic Corynebacterium diphtheriae strains, 9 nontoxigenic C. diphtheriae strains, and 44 strains representing the diversity of pathogens and normal respiratory flora were tested, this real-time PCR assay exhibited 100% sensitivity and specificity. It allowed for the detection of both subunits of the tox gene at 750 times greater sensitivity (2 CFU) than the standard PCR (1,500 CFU). When used directly on specimens collected from patients with clinical diphtheria, one or both subunits of the tox gene were detected in 34 of 36 specimens by using the real-time PCR assay; only 9 specimens were found to be positive by standard PCR. Reamplification by standard PCR and DNA sequencing of the amplification product confirmed all real-time PCR tox-positive reactions. This real-time PCR format is a more sensitive and rapid alternative to standard PCR for detection of the tox gene in clinical material.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.