ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2002
Regulation by glycogen synthase kinase-3beta of the arborization field and maturation of retinotectal projection in zebrafish.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Microtubule-binding protein MAP1B regulates interstitial axon branching of cortical neurons via the tubulin tyrosination cycle.The EMBO journal · 2024Article
- Retrograde labeling of retinal ganglion cells in adult zebrafish with fluorescent dyes.Journal of visualized experiments : JoVE · 2014Article
- Neurogenesis of retinal ganglion cells is not essential to visual functional recovery after optic nerve injury in adult zebrafish.PloS one · 2013Article
- GSK-3 beta inhibits presynaptic vesicle exocytosis by phosphorylating P/Q-type calcium channel and interrupting SNARE complex formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- A TrkB/EphrinA interaction controls retinal axon branching and synaptogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Cell-autonomous TrkB signaling in presynaptic retinal ganglion cells mediates axon arbor growth and synapse maturation during the establishment of retinotectal synaptic connectivity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Vesicular glutamate transport at a central synapse limits the acuity of visual perception in zebrafish.Neuron · 2007Article
- GSK-3 is activated by the tyrosine kinase Pyk2 during LPA1-mediated neurite retraction.Molecular biology of the cell · 2006Article
- Distinct roles of calcineurin-nuclear factor of activated T-cells and protein kinase A-cAMP response element-binding protein signaling in presynaptic differentiation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- In vivo trafficking and targeting of N-cadherin to nascent presynaptic terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
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Authors and funding
4 authors.
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Abstract
The retinotectal projection is one of the best systems to study the molecular basis of synapse formation in the CNS because of the well characterized topographic connections and activity-dependent refinement. Here, we developed a presynaptic neuron-specific gene manipulation system in the zebrafish retinotectal projection in vivo using the nicotinic acetylcholine receptor beta3 (nAChRbeta3) gene promoter. Enhanced green fluorescent protein (EGFP) expression signals in living transgenic zebrafish lines carrying the nAChRbeta3 gene promoter-directed EGFP expression vector visualized the development of entire retinal ganglion cell (RGC) axon projection to the tectum. Microinjection of the nAChRbeta3 gene promoter-driven double-cassette vectors directing the expression of both dominant-negative glycogen synthase kinase-3beta (dnGSK-3beta) and EGFP enabled us to follow the development of individual RGCs and to examine the effect of the molecule on the axonal arborization and maturation of the same neurons in living zebrafish. We found that the expression of the dominant-negative form of zebrafish GSK-3beta suppressed the arborization field of RGC axon terminals in the tectum as estimated by the reduction of arbor branch length and arbor areas. Furthermore, the suppression of GSK-3beta activity increased the size of vesicle-associated membrane protein 2-EGFP puncta in RGC axon terminals at the early stage of innervation to the tectum. These results suggest that GSK-3beta regulates the arborization field and maturation of RGC axon terminals in vivo.
Indexed as
Identifiers
12451132PMC6758727What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.