ArticleThe Journal of general physiology2002
The cation selectivity filter of the bacterial sodium channel, NaChBac.
Article in The Journal of general physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed.
- Structure-function analysis of the lithium-ion selectivity of the voltage-gated sodium channel.The Journal of general physiology · 2026Article
- Absence of Hofmeister Selectivity in Hydrophobic Ion-Exchanger Nanopores.Analytical chemistry · 2025Article
- Bottom-up design of CaNature · 2025Article
- Review
- The chemistry of electrical signaling in sodium channels from bacteria and beyond.Cell chemical biology · 2024Review
- A governance of ion selectivity based on the occupancy of the "beacon" in one- and four-domain calcium and sodium channels.Channels (Austin, Tex.) · 2023Article
- Structure of human NaNature communications · 2023Article
- Switchable NaNature communications · 2022Article
- An open state of a voltage-gated sodium channel involving a π-helix and conserved pore-facing asparagine.Biophysical journal · 2022Article
- Structural Advances in Voltage-Gated Sodium Channels.Frontiers in pharmacology · 2022Review
- Artificial sodium-selective ionic device based on crown-ether crystals with subnanometer pores.Nature communications · 2021Article
- Review
- Brevetoxin and Conotoxin Interactions with Single-Domain Voltage-Gated Sodium Channels from a Diatom and Coccolithophore.Marine drugs · 2021Article
- Application of a Statistical and Linear Response Theory to Multi-Ion NaEntropy (Basel, Switzerland) · 2021Article
- The insights into calcium ion selectivity provided by ancestral prokaryotic ion channels.Biophysics and physicobiology · 2021Article
- In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy.Methods in enzymology · 2021Article
- Changes in Ion Selectivity Following the Asymmetrical Addition of Charge to the Selectivity Filter of Bacterial Sodium Channels.Entropy (Basel, Switzerland) · 2020Article
- A Novel Single-Domain NaPlant physiology · 2020Article
- A native prokaryotic voltage-dependent calcium channel with a novel selectivity filter sequence.eLife · 2020Article
- Bases of Bacterial Sodium Channel Selectivity Among Organic Cations.Scientific reports · 2019Article
26 more citing papers are in PubMed but not listed here.
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The Bacillus halodurans voltage-gated sodium-selective channel (NaChBac) (Ren, D., B. Navarro, H. Xu, L. Yue, Q. Shi, and D.E. Clapham. 2001b. SCIENCE: 294:2372-2375), is an ideal candidate for high resolution structural studies because it can be expressed in mammalian cells and its functional properties studied in detail. It has the added advantage of being a single six transmembrane (6TM) orthologue of a single repeat of mammalian voltage-gated Ca(2+) (Ca(V)) and Na(+) (Na(V)) channels. Here we report that six amino acids in the pore domain (LESWAS) participate in the selectivity filter. Replacing the amino acid residues adjacent to glutamatic acid (E) by a negatively charged aspartate (D; LEDWAS) converted the Na(+)-selective NaChBac to a Ca(2+)- and Na(+)-permeant channel. When additional aspartates were incorporated (LDDWAD), the mutant channel resulted in a highly expressing voltage-gated Ca(2+)-selective conductance.
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