ArticleApplied and environmental microbiology2002
Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 116 citations in OpenAlex.
- Listeria monocytogenes in the seafood industry: Exploring contamination sources, outbreaks, antibiotic susceptibility and genetic diversity.MicrobiologyOpen · 2024Review
- Simultaneous Detection of Four Main Foodborne Pathogens in Ready-to-Eat Food by Using a Simple and Rapid Multiplex PCR (mPCR) Assay.International journal of environmental research and public health · 2022Article
- Microbiological and Chemical Characteristics of Wet Coffee Fermentation Inoculated WithFrontiers in microbiology · 2021Article
- Virulence Characterization ofPathogens (Basel, Switzerland) · 2020Article
- Validation of Predicted Virulence Factors inToxins · 2019Article
- Sourdough authentication: quantitative PCR to detect the lactic acid bacterial microbiota in breads.Scientific reports · 2017Article
- Phage Inactivation of Listeria monocytogenes on San Daniele Dry-Cured Ham and Elimination of Biofilms from Equipment and Working Environments.Microorganisms · 2016Article
- Nucleic acid-based biotechnologies for food-borne pathogen detection using routine time-intensive culture-based methods and fast molecular diagnostics.Food science and biotechnology · 2016Review
- Antimicrobial resistance and virulence signatures of Listeria and Aeromonas species recovered from treated wastewater effluent and receiving surface water in Durban, South Africa.BMC microbiology · 2015Article
- Illumina-based analysis of bacterial diversity related to halophytes Salicornia europaea and Sueada aralocaspica.Journal of microbiology (Seoul, Korea) · 2015Article
- Molecular tools for the selective detection of nine diatom species biomarkers of various water quality levels.International journal of environmental research and public health · 2015Article
- Suppression ofFoods (Basel, Switzerland) · 2013Article
- Municipal wastewater effluents as a source of listerial pathogens in the aquatic milieu of the Eastern Cape Province of South Africa: a concern of public health importance.International journal of environmental research and public health · 2010Article
- Endophytic bacterial diversity in rice (Oryza sativa L.) roots estimated by 16S rDNA sequence analysis.Microbial ecology · 2008Article
- Rapid discrimination of Listeria monocytogenes strains by microtemperature gradient gel electrophoresis.Journal of clinical microbiology · 2006Article
- Reliable and rapid identification of Listeria monocytogenes and Listeria species by artificial neural network-based Fourier transform infrared spectroscopy.Applied and environmental microbiology · 2006Article
- Culture-dependent and -independent methods to investigate the microbial ecology of Italian fermented sausages.Applied and environmental microbiology · 2005Article
- Study of the ecology of fresh sausages and characterization of populations of lactic acid bacteria by molecular methods.Applied and environmental microbiology · 2004Article
- Rapid identification of Listeria species by using restriction fragment length polymorphism of PCR-amplified 23S rRNA gene fragments.Applied and environmental microbiology · 2003Article
- Chemical Composition, Antioxidant and Antibacterial Activity of Bunium persicum, Eucalyptus globulus, and Rose Water on Multidrug-Resistant Listeria Species.Journal of evidence-based integrative medicineArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new molecular approach for the detection and identification of Listeria spp. and Listeria monocytogenes in food is presented here. The method is based on the PCR amplification of a fragment of the iap gene from the five species belonging to the genus and on the analysis of the PCR products obtained by denaturing gradient gel electrophoresis (DGGE). The protocol was first optimized by using strains from international collections. Based on the differences present in the sequences amplified, it was possible to obtain species-specific DGGE migration that allowed fast and easy identification of L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, and L. ivanovii. Moreover, for L. monocytogenes serotypes, partial differentiation was possible. The optimized protocol was used for identification of Listeria strains traditionally isolated from food and for direct detection and identification of Listeria members in food after an overnight enrichment. Identification of 48 food isolates and direct detection of Listeria spp. in 73 food samples show the potential of the method that can be used as a fast screening test to investigate the presence of Listeria spp. and L. monocytogenes in food.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.