ArticleApplied and environmental microbiology2002
Aquaporin expression correlates with freeze tolerance in baker's yeast, and overexpression improves freeze tolerance in industrial strains.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 148 citations in OpenAlex.
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- Root remodeling mechanisms and salt tolerance trade-offs: The roles of HKT1, TMAC2, and TIP2;2 in Arabidopsis.PLoS genetics · 2025Article
- Enhancing freeze-thaw tolerance in baker's yeast: strategies and perspectives.Food science and biotechnology · 2024Review
- Polyextremophile engineering: a review of organisms that push the limits of life.Frontiers in microbiology · 2024Review
- High-throughput RNA sequencing reveals differences between the transcriptomes of the five spore forms of Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen.Stress biology · 2023Article
- A high-throughput yeast approach to characterize aquaporin permeabilities: Profiling the Arabidopsis PIP aquaporin sub-family.Frontiers in plant science · 2023Article
- Protein Structure and Modification of Aquaporins.Advances in experimental medicine and biology · 2023Article
- Improving carotenoid production in recombinant yeast,Engineering in life sciences · 2022Article
- Expression of a COeLife · 2021Article
- Development of Freeze-Thaw Stable Starch through Enzymatic Modification.Foods (Basel, Switzerland) · 2021Article
- Review
- Higher Expression Levels of Aquaporin Family of Proteins in the Kidneys of Arid-Desert LivingFrontiers in physiology · 2019Article
- Quantitative Trait Nucleotides Impacting the Technological Performances of IndustrialFrontiers in genetics · 2019Article
- Temperature dependence of protein-water interactions in a gated yeast aquaporin.Scientific reports · 2017Article
- TheG3 (Bethesda, Md.) · 2017Article
- Comparative transcriptome analysis revealing dormant conidia and germination associated genes in Aspergillus species: an essential role for AtfA in conidial dormancy.BMC genomics · 2016Article
- Article
- Diversity and adaptive evolution of Saccharomyces wine yeast: a review.FEMS yeast research · 2015Review
- Can Stabilization and Inhibition of Aquaporins Contribute to Future Development of Biomimetic Membranes?Membranes · 2015Review
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little information is available about the precise mechanisms and determinants of freeze resistance in baker's yeast, Saccharomyces cerevisiae. Genomewide gene expression analysis and Northern analysis of different freeze-resistant and freeze-sensitive strains have now revealed a correlation between freeze resistance and the aquaporin genes AQY1 and AQY2. Deletion of these genes in a laboratory strain rendered yeast cells more sensitive to freezing, while overexpression of the respective genes, as well as heterologous expression of the human aquaporin gene hAQP1, improved freeze tolerance. These findings support a role for plasma membrane water transport activity in determination of freeze tolerance in yeast. This appears to be the first clear physiological function identified for microbial aquaporins. We suggest that a rapid, osmotically driven efflux of water during the freezing process reduces intracellular ice crystal formation and resulting cell damage. Aquaporin overexpression also improved maintenance of the viability of industrial yeast strains, both in cell suspensions and in small doughs stored frozen or submitted to freeze-thaw cycles. Furthermore, an aquaporin overexpression transformant could be selected based on its improved freeze-thaw resistance without the need for a selectable marker gene. Since aquaporin overexpression does not seem to affect the growth and fermentation characteristics of yeast, these results open new perspectives for the successful development of freeze-resistant baker's yeast strains for use in frozen dough applications.
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