ArticleNature biotechnology2002
Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation.
Article in Nature biotechnology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 114 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Three Month, Open-label, Single-arm Trial Evaluating the Safety and Pharmacokinetics of Oral Lithium in Patients Diagnosed With Chronic Spinal Cord Injury
A Randomized Controlled Phase II, Two-Arm Study of Umbilical Cord Blood Cell Transplant (MC001) Into Injured Spinal Cord Followed by the Locomotor Training for Patients with Chronic Complete Spinal Cord Injuries (SCI).
Who cites it
114 citing papers in PubMed, 326 citations in OpenAlex.
- The prognostic value of secreted frizzled-related protein 2 in elderly patients with heart failure: a single-center retrospective study.Scientific reports · 2025Article
- Transcriptional Regulation of Lipid Metabolism by Wnt Signaling and Hox Protein Cues.Advances in experimental medicine and biology · 2025Review
- Functional dissection of PRC1 subunits RYBP and YAF2 during neural differentiation of embryonic stem cells.Nature communications · 2023Article
- TGF-β and SHH Regulate Pluripotent Stem Cell Differentiation into Brain Microvascular Endothelial Cells in Generating an In Vitro Blood-Brain Barrier Model.Bioengineering (Basel, Switzerland) · 2023Article
- Human 3D brain organoids: steering the demolecularization of brain and neurological diseases.Cell death discovery · 2023Review
- Reproducible generation of human retinal ganglion cells from banked retinal progenitor cells: analysis of target recognition and IGF-1-mediated axon regeneration.Frontiers in cell and developmental biology · 2023Article
- Deciphering the Transcriptional Landscape of Human Pluripotent Stem Cell-Derived GnRH Neurons: The Role of Wnt Signaling in Patterning the Neural Fate.Stem cells (Dayton, Ohio) · 2022Article
- The Effects of Co-Culture of Embryonic Stem Cells with Neural Stem Cells on Differentiation.Current issues in molecular biology · 2022Article
- Reconstruction of dynamic regulatory networks reveals signaling-induced topology changes associated with germ layer specification.Stem cell reports · 2022Article
- Peripheral Blood Circular RNAs as a Biomarker for Major Depressive Disorder and Prediction of Possible Pathways.Frontiers in neuroscience · 2022Article
- Integrated loss- and gain-of-function screens define a core network governing human embryonic stem cell behavior.Genes & development · 2021Article
- Deciphering and reconstitution of positional information in the human brain development.Cell regeneration (London, England) · 2021Review
- Strategy to Establish Embryo-Derived Pluripotent Stem Cells in Cattle.International journal of molecular sciences · 2021Review
- Article
- Cadherins in early neural development.Cellular and molecular life sciences : CMLS · 2021Review
- SALL4 controls cell fate in response to DNA base composition.Molecular cell · 2021Article
- Inverse Associations Between Circulating Secreted Frizzled Related Protein 2 (sFRP2) and Cardiometabolic Risk Factors.Frontiers in cardiovascular medicine · 2021Article
- The Distinct Role of Tcfs and Lef1 in the Self-Renewal or Differentiation of Mouse Embryonic Stem Cells.International journal of stem cells · 2020Article
- Recapitulating developmental mechanisms for retinal regeneration.Progress in retinal and eye research · 2020Review
- Multiple Roles of sFRP2 in Cardiac Development and Cardiovascular Disease.International journal of biological sciences · 2020Review
54 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multilineage differentiation capacity of mouse embryonic stem (ES) cells offers a potential testing platform for gene products that mediate mammalian lineage determination and cellular specialization. Identification of such differentiation regulators is crucial to harnessing ES cells for pharmaceutical discovery and cell therapy. Here we describe the use of episomal expression technology for functional evaluation of cDNA clones during ES-cell differentiation in vitro. Several candidate cDNAs identified by subtractive cloning and expression profiling were introduced into ES cells in episomal expression constructs. Subsequent differentiation revealed that the Wnt antagonist Sfrp2 stimulates production of neural progenitors. The significance of this observation was substantiated by forced expression of Wnt-1 and treatment with lithium chloride, both of which inhibit neural differentiation. These findings reveal the importance of Wnt signaling in regulating ES-cell lineage diversification. More generally, this study establishes a path for rapid and direct validation of candidate genes in ES cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.