ArticleProceedings of the National Academy of Sciences of the United States of America2002
A highly Ca2+-sensitive pool of vesicles is regulated by protein kinase C in adrenal chromaffin cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 88 citations in OpenAlex.
- Roles for PKC signaling in chromaffin cell exocytosis.Biophysical journal · 2025Article
- Cellular mechanisms underlying pituitary adenylate cyclase activating polypeptide-stimulated secretion in the adrenal medulla.Biochemical Society transactions · 2024Review
- Mitochondrial hydrogen peroxide positively regulates neuropeptide secretion during diet-induced activation of the oxidative stress response.Nature communications · 2021Article
- SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.JCI insight · 2020Article
- Calcium dependence of spontaneous neurotransmitter release.Journal of neuroscience research · 2018Review
- Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells.Cell calcium · 2017Review
- Article
- PKC enhances the capacity for secretion by rapidly recruiting covert voltage-gated Ca2+ channels to the membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- On depolarization-evoked exocytosis as a function of calcium entry: possibilities and pitfalls.Biophysical journal · 2011Article
- Two distinct vesicle pools for depolarization-induced exocytosis in somata of dorsal root ganglion neurons.The Journal of physiology · 2011Article
- Vesicle pools: lessons from adrenal chromaffin cells.Frontiers in synaptic neuroscience · 2011Article
- PKC epsilon facilitates recovery of exocytosis after an exhausting stimulation.Pflugers Archiv : European journal of physiology · 2009Article
- Unifying concepts in stimulus-secretion coupling in endocrine cells and some implications for therapeutics.Advances in physiology education · 2009Review
- Newcomer insulin secretory granules as a highly calcium-sensitive pool.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Effects of phorbol ester on vesicle dynamics as revealed by total internal reflection fluorescence microscopy.Pflugers Archiv : European journal of physiology · 2008Article
- PKC theta activity maintains normal quantal size in chromaffin cells.Journal of neurochemistry · 2008Article
- Dopamine inhibits basal prolactin release in pituitary lactotrophs through pertussis toxin-sensitive and -insensitive signaling pathways.Endocrinology · 2008Article
- Synaptotagmin-1 and -7 are functionally overlapping Ca2+ sensors for exocytosis in adrenal chromaffin cells.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Increased secretory capacity of mouse adrenal chromaffin cells by chronic intermittent hypoxia: involvement of protein kinase C.The Journal of physiology · 2007Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have used flash photolysis of caged Ca2+ and membrane capacitance measurements to probe exocytosis in chromaffin cells at low concentrations of intracellular Ca2+ ([Ca2+]i) (<10 microM). We observed a small pool of granules that is more sensitive to [Ca2+]i than the previously described "readily releasable pool." Upon activation of PKC, this "highly Ca2+-sensitive pool" is enhanced in size to a greater extent than the readily releasable pool but is eliminated upon expression of a C-terminal deletion mutant (Delta9) of synaptosome-associated protein of 25 kDa (SNAP-25). Thus, in chromaffin cells, PKC enhances exocytosis both by increasing the number of readily releasable vesicles and by shifting vesicles to a highly Ca2+-sensitive state, enabling exocytosis at sites relatively distant from Ca2+ channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.