ArticleThe Journal of cell biology2002
Localized suppression of RhoA activity by Tyr31/118-phosphorylated paxillin in cell adhesion and migration.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 175 citations in OpenAlex.
- TLR4-paxillin-Rac1 signaling mediates LPS-induced dysregulation of keratinocyte function.iScience · 2026Article
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- CYRI-B loss promotes enlarged mature focal adhesions and restricts microtubule and ERC1 access to the cell leading edge.Journal of cell science · 2025Article
- Paxillin promotes the invasion and migration of polyploid giant cancer cells with daughter cells after arsenic trioxide treatment by regulating the expression of cathepsin B/D.Journal of Cancer · 2025Article
- TLK1>Nek1 Axis Promotes Nuclear Retention and Activation of YAP with Implications for Castration-Resistant Prostate Cancer.Cancers · 2024Article
- Hypoxia-induced YAP activation and focal adhesion turnover to promote cell migration in mesenchymal TNBC cells.Cancer medicine · 2023Article
- Lack of Paxillin phosphorylation promotes single-cell migration in vivo.The Journal of cell biology · 2023Article
- The TLK1-MK5 Axis Regulates Motility, Invasion, and Metastasis of Prostate Cancer Cells.Cancers · 2022Article
- Metastasis and MAPK Pathways.International journal of molecular sciences · 2022Review
- Paxillin promotes breast tumor collective cell invasion through maintenance of adherens junction integrity.Molecular biology of the cell · 2022Article
- A Layered View on Focal Adhesions.Biology · 2021Review
- Review
- Paxillin Is Required for Proper Spinal Motor Axon Growth into the Limb.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- FAK-Dependent Cell Motility and Cell Elongation.Cells · 2020Review
- Paxillin family of focal adhesion adaptor proteins and regulation of cancer cell invasion.International review of cell and molecular biology · 2020Article
- Upregulation of ARHGAP30 attenuates pancreatic cancer progression by inactivating the β-catenin pathway.Cancer cell international · 2020Article
- Regulation of Rho GTPase activity at the leading edge of migrating cells by p190RhoGAP.Small GTPases · 2019Review
- Differential Regulation of Adhesion and Phagocytosis of Resting and Activated Microglia by Dopamine.Frontiers in cellular neuroscience · 2018Article
- Proteome Analysis of Human Follicular Thyroid Cancer Cells Exposed to the Random Positioning Machine.International journal of molecular sciences · 2017Article
- Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor.Methods in enzymology · 2017Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RhoA activity is transiently inhibited at the initial phase of integrin engagement, when Cdc42- and/or Rac1-mediated membrane spreading and ruffling predominantly occur. Paxillin, an integrin-assembly protein, has four major tyrosine phosphorylation sites, and the phosphorylation of Tyr31 and Tyr118 correlates with cell adhesion and migration. We found that mutation of Tyr31/118 caused enhanced activation of RhoA and premature formation of stress fibers with substantial loss of efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. These phenotypes were similar to those induced by RhoA(G14V) in parental cells, and could be abolished by expression of RhoA(T19N), Rac1(G12V), or p190RhoGAP in the mutant-expressing cells. Phosphorylated Tyr31/118 was found to bind to two src homology (SH)2 domains of p120RasGAP, with coprecipitation of endogenous paxillin with p120RasGAP. p190RhoGAP is known to be a major intracellular binding partner for the p120RasGAP SH2 domains. We found that Tyr31/118-phosphorylated paxillin competes with p190RhoGAP for binding to p120RasGAP, and provides evidence that p190RhoGAP freed from p120RasGAP efficiently suppresses RhoA activity during cell adhesion. We conclude that Tyr31/118-phosphorylated paxillin serves as a template for the localized suppression of RhoA activity and is necessary for efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.