ArticleThe Journal of cell biology2002
Cytoplasmic linker proteins promote microtubule rescue in vivo.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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Who cites it
109 citing papers in PubMed, 245 citations in OpenAlex.
- Tubulin autoregulation tunes microtubule dynamics to support multicellular architecture and viability.Nature communications · 2026Article
- CLASP2 promotes repair of kinesin-1 damage to the microtubule lattice.bioRxiv : the preprint server for biology · 2026Article
- Modulation of microtubule dynamics by monovalent ions.PNAS nexus · 2025Article
- Liquid-liquid phase separation of microtubule-binding proteins in the regulation of spindle assembly.Cell proliferation · 2024Review
- Autophagy initiation triggers p150Cellular and molecular life sciences : CMLS · 2024Article
- RAB11A and RAB11B control mitotic spindle function in intestinal epithelial progenitor cells.EMBO reports · 2023Article
- Phase separation of +TIP networks regulates microtubule dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microtubule plus-end tracking proteins: novel modulators of cardiac sodium channels and arrhythmogenesis.Cardiovascular research · 2023Article
- The motor domain of the kinesin Kip2 promotes microtubule polymerization at microtubule tips.The Journal of cell biology · 2023Article
- Microtubule detyrosination drives symmetry breaking to polarize cells for directed cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Modeling reveals cortical dynein-dependent fluctuations in bipolar spindle length.Biophysical journal · 2021Article
- α-tubulin tail modifications regulate microtubule stability through selective effector recruitment, not changes in intrinsic polymer dynamics.Developmental cell · 2021Article
- Persistent growth of microtubules at low density.Molecular biology of the cell · 2021Article
- Article
- Article
- Tubulin islands containing slowly hydrolyzable GTP analogs regulate the mechanism and kinetics of microtubule depolymerization.Scientific reports · 2020Article
- Stress-induced phosphorylation of CLIP-170 by JNK promotes microtubule rescue.The Journal of cell biology · 2020Article
- NMR resonance assignment and structure prediction of the C-terminal domain of the microtubule end-binding protein 3.PloS one · 2020Article
- Microtubules in cell migration.Essays in biochemistry · 2019Review
49 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The role of plus end-tracking proteins in regulating microtubule (MT) dynamics was investigated by expressing a dominant negative mutant that removed endogenous cytoplasmic linker proteins (CLIPs) from MT plus ends. In control CHO cells, MTs exhibited asymmetric behavior: MTs persistently grew toward the plasma membrane and displayed frequent fluctuations of length near the cell periphery. In the absence of CLIPs, the microtubule rescue frequency was reduced by sevenfold. MT behavior became symmetrical, consisting of persistent growth and persistent shortening. Removal of CLIPs also caused loss of p150Glued but not CLIP-associating protein (CLASP2) or EB1. This result raised the possibility that the change in dynamics was a result of the loss of either CLIPs or p150Glued. To distinguish between these possibilities, we performed rescue experiments. Normal MT dynamics were restored by expression of the CLIP-170 head domain, but p150Glued was not recruited back to MT plus ends. Expression of p150Glued head domain only partially restored MT dynamics. We conclude that the CLIP head domain is sufficient to alter MT dynamics either by itself serving as a rescue factor or indirectly by recruiting a rescue factor. By promoting a high rescue frequency, CLIPs provide a mechanism by which MT plus ends may be concentrated near the cell margin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.