ArticleThe Journal of cell biology2002
K-loop insertion restores microtubule depolymerizing activity of a "neckless" MCAK mutant.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 100 citations in OpenAlex.
- KIF2C/MCAK a prognostic biomarker and its oncogenic potential in malignant progression, and prognosis of cancer patients: a systematic review and meta-analysis as biomarker.Critical reviews in clinical laboratory sciences · 2024Pooled it
- A kinesin-13 family kinesin in Trypanosoma brucei regulates cytokinesis and cytoskeleton morphogenesis by promoting microtubule bundling.PLoS pathogens · 2024Article
- A synthetic ancestral kinesin-13 depolymerizes microtubules faster than any natural depolymerizing kinesin.Open biology · 2022Article
- These motors were made for walking.Protein science : a publication of the Protein Society · 2020Review
- Functional characterization of MCAK/Kif2C cancer mutations using high-throughput microscopic analysis.Molecular biology of the cell · 2020Article
- Kinesin-13 and Kinesin-8 Function during Cell Growth and Division in the MossThe Plant cell · 2020Article
- Parts list for a microtubule depolymerising kinesin.Biochemical Society transactions · 2018Review
- KNL1 Binding to PP1 and Microtubules Is Mutually Exclusive.Structure (London, England : 1993) · 2018Article
- Ternary complex of Kif2A-bound tandem tubulin heterodimers represents a kinesin-13-mediated microtubule depolymerization reaction intermediate.Nature communications · 2018Article
- Poly(ADP-ribose) mediates asymmetric division of mouse oocyte.Cell research · 2018Article
- Discovery of thalicthuberine as a novel antimitotic agent from nature that disrupts microtubule dynamics and induces apoptosis in prostate cancer cells.Cell cycle (Georgetown, Tex.) · 2018Article
- β-tubulin carboxy-terminal tails exhibit isotype-specific effects on microtubule dynamics in human gene-edited cells.Life science alliance · 2018Article
- Insight into microtubule disassembly by kinesin-13s from the structure of Kif2C bound to tubulin.Nature communications · 2017Article
- MCAK-mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin-II contractility.Molecular biology of the cell · 2017Article
- Article
- The far C-terminus of MCAK regulates its conformation and spindle pole focusing.Molecular biology of the cell · 2016Article
- Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins.Molecular biology of the cell · 2016Article
- Article
- Nucleotide exchange in dimeric MCAK induces longitudinal and lateral stress at microtubule ends to support depolymerization.Structure (London, England : 1993) · 2014Article
- Aurora B inhibits MCAK activity through a phosphoconformational switch that reduces microtubule association.Current biology : CB · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Unlike most kinesins, mitotic centromere-associated kinesin (MCAK) does not translocate along the surface of microtubules (MTs), but instead depolymerizes them. Among the motile kinesins, refinements that are unique for specific cellular functions, such as directionality and processivity, are under the control of a "neck" domain adjacent to the ATP-hydrolyzing motor domain. Despite its apparent lack of motility, MCAK also contains a neck domain. We found that deletions and alanine substitutions of highly conserved positively charged residues in the MCAK neck domain significantly reduced MT depolymerization activity. Furthermore, substitution of MCAK's neck domain with either the positively charged KIF1A K-loop or poly-lysine rescues the loss of MT-depolymerizing activity observed in the neckless MCAK mutant. We propose that the neck, analogously to the K-loop, interacts electrostatically with the tubulin COOH terminus to permit diffusional translocation of MCAK along the surface of MTs. This weak-binding interaction may also play an important role in processivity of MCAK-induced MT depolymerization.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.