ArticleThe Biochemical journal2003
Genomic organization and transcriptional analysis of the human l-glutaminase gene.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- HuR controls glutaminase RNA metabolism.Nature communications · 2024Article
- Two Faces of Glutaminase GLS2 in Carcinogenesis.Cancers · 2023Review
- High-Throughput Screening Reveals New Glutaminase Inhibitor Molecules.ACS pharmacology & translational science · 2021Article
- Dual inhibition of glutaminase and carnitine palmitoyltransferase decreases growth and migration of glutaminase inhibition-resistant triple-negative breast cancer cells.The Journal of biological chemistry · 2019Article
- Glutamate and Brain Glutaminases in Drug Addiction.Neurochemical research · 2017Review
- NF-Y activates genes of metabolic pathways altered in cancer cells.Oncotarget · 2016Article
- Glutaminase 2 negatively regulates the PI3K/AKT signaling and shows tumor suppression activity in human hepatocellular carcinoma.Oncotarget · 2014Article
- Both GLS silencing and GLS2 overexpression synergize with oxidative stress against proliferation of glioma cells.Journal of molecular medicine (Berlin, Germany) · 2014Article
- STAT1 regulates human glutaminase 1 promoter activity through multiple binding sites in HIV-1 infected macrophages.PloS one · 2013Article
- Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.PloS one · 2012Article
- Interferon-α regulates glutaminase 1 promoter through STAT1 phosphorylation: relevance to HIV-1 associated neurocognitive disorders.PloS one · 2012Article
- Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Co-expression of glutaminase K and L isoenzymes in human tumour cells.The Biochemical journal · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals, glutaminase (GA) is expressed in most tissues, but the regulation of organ-specific expression is largely unknown. Therefore, as an essential step towards studying the regulation of GA expression, the human liver-type GA (hLGA) gene has been characterized. LGA genomic sequences were isolated using the genome walking technique. Analysis and comparison of these sequences with two LGA cDNA clones and the Human Genome Project database, allowed the determination of the genomic organization of the LGA gene. The gene has 18 exons and is approx. 18 kb long. All exon/intron junction sequences conform to the GT/AG rule. Progressive deletion analysis of LGA promoter-luciferase constructs indicated that the core promoter is located between nt -141 and +410, with several potential regulatory elements: CAAT, GC, TATA-like, Ras-responsive element binding protein and specificity protein 1 (Sp1) sites. The minimal promoter was mapped within +107 and +410, where only an Sp1 binding site is present. Mutation experiments suggested that two CAAT recognition elements near the transcription-initiation site (-138 and -87), play a crucial role for optimal promoter activity. Electrophoretic mobility-shift assays confirmed the importance of CAAT- and TATA-like boxes to enhance basal transcription, and demonstrated that HNF-1 motif is a significant distal element for transcriptional regulation of the hLGA gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.