ArticleProceedings of the National Academy of Sciences of the United States of America2002
Artificial human telomeres from DNA nanocircle templates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 40 citations in OpenAlex.
- Article
- The Discovery of Rolling Circle Amplification and Rolling Circle Transcription.Accounts of chemical research · 2016Article
- Maintenance of very long telomeres by recombination in the Kluyveromyces lactis stn1-M1 mutant involves extreme telomeric turnover, telomeric circles, and concerted telomeric amplification.Molecular and cellular biology · 2012Article
- Visualization of long human telomere mimics by single-molecule fluorescence imaging.The journal of physical chemistry. B · 2008Article
- In situ detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS.BMC biotechnology · 2007Article
- Mutation of DNA polymerase beta in esophageal carcinoma of different regions.World journal of gastroenterology · 2005Article
- Extrachromosomal telomeric circles contribute to Rad52-, Rad50-, and polymerase delta-mediated telomere-telomere recombination in Saccharomyces cerevisiae.Eukaryotic cell · 2005Article
- Efficient isothermal expansion of human telomeric and minisatellite repeats by Thermococcus litoralis DNA polymerase.Nucleic acids research · 2005Article
- Small circular DNAs for synthesis of the human telomere repeat: varied sizes, structures and telomere-encoding activities.Nucleic acids research · 2004Article
- Factors influencing the recombinational expansion and spread of telomeric tandem arrays in Kluyveromyces lactis.Eukaryotic cell · 2003Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human telomerase is a reverse-transcriptase enzyme that synthesizes the multikilobase repeating hexamer telomere sequence (TTAGGG)n at the ends of chromosomes. Here we describe a designed approach to mimicry of telomerase, in which synthetic DNA nanocircles act as essentially infinite catalytic templates for efficient synthesis of long telomeres by DNA polymerase enzymes. Results show that the combination of a nanocircle and a DNA polymerase gives a positive telomere-repeat amplification protocol assay result for telomerase activity, and similar to the natural enzyme, it is inhibited by a known telomerase inhibitor. We show that artificial telomeres can be engineered on human chromosomes by this approach. This strategy allows for the preparation of synthetic telomeres for biological and structural study of telomeres and proteins that interact with them, and it raises the possibility of telomere engineering in cells without expression of telomerase itself. Finally, the results provide direct physical support for a recently proposed rolling-circle mechanism for telomerase-independent telomere elongation.
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