ArticleWorld journal of gastroenterology2002
Effects of tachyplesin on proliferation and differentiation of human hepatocellular carcinoma SMMC-7721 cells.
Article in World journal of gastroenterology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Cationic antimicrobial peptide CC34 potential anticancer and apoptotic induction on cancer cells.Amino acids · 2025Article
- Anticancer Mechanisms and Potential Anticancer Applications of Antimicrobial Peptides and Their Nano Agents.International journal of nanomedicine · 2024Review
- Natural Anticancer Peptides from Marine Animal Species: Evidence from In Vitro Cell Model Systems.Cancers · 2023Review
- Anti-Cancer Peptides: Status and Future Prospects.Molecules (Basel, Switzerland) · 2023Review
- The role of lysosomal peptidases in glioma immune escape: underlying mechanisms and therapeutic strategies.Frontiers in immunology · 2023Review
- Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties.International journal of molecular sciences · 2019Article
- Exploration of anti-cancer effects and mechanisms of Zuo-Jin-Wan and its alkaloid components in vitro and in orthotopic HepG2 xenograft immunocompetent mice.BMC complementary and alternative medicine · 2017Article
- Article
- Genomic and functional analysis of the toxic effect of tachyplesin I on the embryonic development of zebrafish.Computational and mathematical methods in medicine · 2014Article
- Retrocyclins and their activity against HIV-1.Cellular and molecular life sciences : CMLS · 2011Review
- Induced differentiation of hepatocellular carcinoma by natural products.African journal of traditional, complementary, and alternative medicines : AJTCAM · 2008Article
- Studies on anticancer activities of antimicrobial peptides.Biochimica et biophysica acta · 2008Review
- Growth arrest and apoptosis of human hepatocellular carcinoma cells induced by hexamethylene bisacetamide.World journal of gastroenterology · 2004Article
- Effects of vitamin E succinate on the expression of Fas and PCNA proteins in human gastric carcinoma cells and its clinical significance.World journal of gastroenterology · 2004Article
- Effect of staurosporine on cycle of human gastric cancer cells.World journal of gastroenterology · 2004Article
- Effects of tachyplesin on the regulation of cell cycle in human hepatocarcinoma SMMC-7721 cells.World journal of gastroenterology · 2003Article
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5 authors.
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Abstract
aimTo investigate the antitumor activities of tachyplesin on human hepatocellular carcinoma (HCC) cells.
methodsTachyplesin, isolated from acid extracts of Chinese horseshoe crab (Tachypleus tridentatus) hemocytes, was used to treat the human HCC cell line SMMC-7721. Effects of tachyplesin on the proliferation of SMMC-7721 cells were measured with trypan blue dye exclusion test and HE staining. The morphology and ultrastructure of the cells were examined by light microscopy and transmission electron microscopy, respectively. The activities of gamma-glutamyltransferase (gamma-GT) and tyrosine aminotransferase (TAT) were assayed with biochemical methods. The levels of alpha fetoprotein (alpha-FP), proliferating cell nuclear antigen (PCNA), p21( WAF1/CIP1) and c-myc were examined by immunocytochemistry.
resultsAfter treatment with tachyplesin 3.0 mg/L, the proliferation of SMMC-7721 cells was inhibited significantly, with the cell growth inhibitory rate amounted to 55.57 % and the maximum cell mitotic index declined by 43.68 %. The morphology and ultrastructure underwent restorational alteration. The activity of gamma-GT declined while TAT activity increased obviously, and the levels of alpha-FP and PCNA decreased. Moreover, the expression of p21(WAF1/CIP1) protein was up-regulated and that of c-myc protein was down-regulated.
conclusionTachyplesin could effectively inhibit the proliferation of hepatocarcinoma cells, reverse the malignant morphological and ultrastructural characteristics, alter the levels of enzymes and antigens, regulate the expression of differentiation-associated oncogene and tumor suppressor gene, and induce hepatocarcinama cell differentiation.
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