ArticleJournal of virology2002
Requirement for vacuolar H+ -ATPase activity and Ca2+ gradient during entry of rotavirus into MA104 cells.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Fatty acid 2-hydroxylase facilitates rotavirus uncoating and endosomal escape.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Characterization of the rotavirus assembly pathway in situ using cryoelectron tomography.Cell host & microbe · 2023Article
- N-Glycosylation of Rotavirus NSP4 Protein Affects Viral Replication and Pathogenesis.Journal of virology · 2023Article
- Quercetin, a flavonoid, combats rotavirus infection by deactivating rotavirus-induced pro-survival NF-κB pathway.Frontiers in microbiology · 2022Article
- Calcium Ions Signaling: Targets for Attack and Utilization by Viruses.Frontiers in microbiology · 2022Review
- Antiviral Properties of Human Milk.Microorganisms · 2021Review
- Host receptors: the key to establishing cells with broad viral tropism for vaccine production.Critical reviews in microbiology · 2020Review
- Activation of PI3K, Akt, and ERK during early rotavirus infection leads to V-ATPase-dependent endosomal acidification required for uncoating.PLoS pathogens · 2018Article
- Inflammatory and oxidative stress in rotavirus infection.World journal of virology · 2016Review
- Characterization and Transferring of Human Rotavirus Double-Layered Particles in MA104 Cells.Jundishapur journal of microbiology · 2014Article
- Genome-wide RNAi screen reveals a role for the ESCRT complex in rotavirus cell entry.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Cholesterol dependence of collagen and echovirus 1 trafficking along the novel α2β1 integrin internalization pathway.PloS one · 2013Article
- Rhesus rotavirus trafficking during entry into MA104 cells is restricted to the early endosome compartment.Journal of virology · 2012Article
- Structural insights into the coupling of virion assembly and rotavirus replication.Nature reviews. Microbiology · 2012Review
- Rhesus rotavirus entry into a polarized epithelium is endocytosis dependent and involves sequential VP4 conformational changes.Journal of virology · 2011Article
- Different rotavirus strains enter MA104 cells through different endocytic pathways: the role of clathrin-mediated endocytosis.Journal of virology · 2010Article
- Effect of mutations in VP5 hydrophobic loops on rotavirus cell entry.Journal of virology · 2010Article
- Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Learning from the viral journey: how to enter cells and how to overcome intracellular barriers to reach the nucleus.The AAPS journal · 2009Review
- Expression of nonstructural rotavirus protein NSP4 mimics Ca2+ homeostasis changes induced by rotavirus infection in cultured cells.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism by which rotavirus and other nonenveloped viruses enter the cell is still not clear. We have proposed an endocytosis model where the critical step for virus uncoating and membrane permeabilization is the decrease in Ca(2+) concentration in the endosome. In this paper, we monitored rotavirus entry by measuring alpha-sarcin-rotavirus coentry and infectivity in MA104 cells. The participation of endocytosis, acidification, and endosomal Ca(2+) concentration on virus entry was studied by inhibiting the endosomal H(+)-ATPase with bafilomycin A1 and/or increasing the extracellular calcium reservoir by addition of 10 mM CaEGTA. Rotavirus-alpha-sarcin coentry was inhibited by bafilomycin A1 and by addition of 10 mM CaEGTA. These effects were additive. These substances induced a significant inhibition of infectivity without affecting virus binding and postentry steps. These results are compatible with the interpretation that bafilomycin A1 and CaEGTA block rotavirus penetration from the endosome into the cytoplasm and support our hypothesis of a Ca(2+)-dependent endocytosis model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.