ArticleInfection and immunity2002
Purification and partial characterization of a 32-kilodalton sialic acid-specific lectin from Aspergillus fumigatus.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 59 citations in OpenAlex.
- Aspergillus fumigatus biofilms: Toward understanding how growth as a multicellular network increases antifungal resistance and disease progression.PLoS pathogens · 2021Review
- Aspergillosis and the role of mucins in cystic fibrosis.Pediatric pulmonology · 2017Review
- Interactions of Aspergillus fumigatus Conidia with Airway Epithelial Cells: A Critical Review.Frontiers in microbiology · 2016Review
- Article
- A soluble fucose-specific lectin from Aspergillus fumigatus conidia--structure, specificity and possible role in fungal pathogenicity.PloS one · 2013Article
- Opportunistic invasive fungal pathogen Macrophomina phaseolina prognosis from immunocompromised humans to potential mitogenic RBL with an exceptional and novel antitumor and cytotoxic effect.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Review
- Purification and characterization of a mitogenic lectin from cephalosporium, a pathogenic fungus causing mycotic keratitis.Biochemistry research international · 2010Article
- Steady state and time resolved fluorescence quenching and chemical modification studies of a lectin from endophytic fungus Fusarium solani.Journal of fluorescence · 2010Article
- Optimization of culture conditions and characterization of a new lectin from Aspergillus niger.Indian journal of microbiology · 2009Article
- Melanin is an essential component for the integrity of the cell wall of Aspergillus fumigatus conidia.BMC microbiology · 2009Article
- Purification and partial characterization of a Paracoccidioides brasiliensis protein with capacity to bind to extracellular matrix proteins.Infection and immunity · 2005Article
- Lectin activity in mycelial extracts of Fusarium species.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adherence of the opportunistic fungus Aspergillus fumigatus to the extracellular matrix components is considered a crucial step in the establishment of the infection. Given the high carbohydrate content of these glycoproteins and the role of carbohydrate-protein interactions in numerous adherence processes, the presence of a lectin in A. fumigatus was investigated. Different fungal extracts obtained by sonication or grinding in liquid nitrogen from resting or swollen conidia, as well as from germ tubes and mycelium, were tested by hemagglutination assays using rabbit erythrocytes. A lectin activity was recovered in all the extracts tested. However, sonication of resting conidia resulted in the highest specific activity. Purification of the lectin was achieved by gel filtration followed by ion-exchange and hydrophobic-interaction chromatographies. Analysis of the purified lectin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed an apparent molecular mass of 32 kDa, which is similar to that of the alkaline protease already identified from different strains of A. fumigatus. However, as evidenced by the use of an alkaline protease-deficient mutant, the two activities were supported by distinct proteins. In addition, hemagglutination inhibition experiments using different saccharides and glycoproteins demonstrated the specificity of the lectin for sialic acid residues. Together these results suggest that this lectin may contribute to the attachment of conidia to the extracellular matrix components through the recognition of the numerous terminal sialic acid residues of their carbohydrate chains.
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