ArticleInfection and immunity2002
Identification of a fibronectin-binding protein from Staphylococcus epidermidis.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 153 citations in OpenAlex.
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- A new host cell internalisation pathway for SadA-expressing staphylococci triggered by excreted neurochemicals.Cellular microbiology · 2019Article
- GeneHunt for rapid domain-specific annotation of glycoside hydrolases.Scientific reports · 2019Article
- Bap and Cell Surface Hydrophobicity Are Important Factors inFrontiers in microbiology · 2019Article
- Complete genome sequencing of three human clinical isolates of Staphylococcus caprae reveals virulence factors similar to those of S. epidermidis and S. capitis.BMC genomics · 2018Article
- Staphylococcal Osteomyelitis: Disease Progression, Treatment Challenges, and Future Directions.Clinical microbiology reviews · 2018Review
- Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolates from neonates.BMC microbiology · 2017Article
- Staphylococcal Adhesion and Host Cell Invasion: Fibronectin-Binding and Other Mechanisms.Frontiers in microbiology · 2017Review
- Pathogenic Mechanisms and Host Interactions inFrontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus epidermidis has been reported to bind to a number of host cell extracellular matrix proteins, including fibronectin. Here we report the identification of a fibronectin-binding protein from S. epidermidis. A phage display library of S. epidermidis genomic DNA was constructed and panned against immobilized fibronectin. A number of phagemid clones containing overlapping inserts were identified, and one of these clones, pSE109FN, contained a 1.4-kb insert. Phage pSE109FN was found to bind to fibronectin but not to collagen, fibrinogen, laminin, or vitronectin. However, pSE109FN also bound to heparin, hyaluronate, and plasminogen, although to a lesser extent than it bound to fibronectin. Analysis of The Institute for Genomic Research S. epidermidis genome sequence database revealed a 1.85-kb region within a putative 30.5-kb open reading frame, to which the overlapping DNA inserts contained within the fibronectin-binding phagemids mapped. We have designated the gene encoding the fibronectin-binding domain embp. A recombinant protein, Embp32, which encompassed the fibronectin-binding domain of Embp, blocked the binding of S. epidermidis, but not the binding of Staphylococcus aureus, to fibronectin. In contrast, a recombinant protein, FnBPB[D1-D4], spanning the fibronectin-binding domain of the S. aureus fibronectin-binding protein FnBPB, blocked binding of S. aureus to fibronectin but had a negligible effect on the binding of S. epidermidis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.