ArticleInfection and immunity2002
The abundant polyadenylated transcript 2 DNA sequence of the pathogenic protozoan parasite Entamoeba histolytica represents a nonautonomous non-long-terminal-repeat retrotransposon-like element which is absent in the closely related nonpathogenic species Entamoeba dispar.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Exploring the complexity of genome size reduction in angiosperms.Plant molecular biology · 2024Review
- The non-LTR retrotransposons of Entamoeba histolytica: genomic organization and biology.Molecular genetics and genomics : MGG · 2022Review
- A genomewide overexpression screen identifies genes involved in the phosphatidylinositol 3-kinase pathway in the human protozoan parasite Entamoeba histolytica.Eukaryotic cell · 2014Article
- Genomic distribution of SINEs in Entamoeba histolytica strains: implication for genotyping.BMC genomics · 2013Article
- RNA-Mediated Gene Duplication and Retroposons: Retrogenes, LINEs, SINEs, and Sequence Specificity.International journal of evolutionary biology · 2013Article
- Recombinant SINEs are formed at high frequency during induced retrotransposition in vivo.Nature communications · 2012Article
- Differential distribution of a SINE element in the Entamoeba histolytica and Entamoeba dispar genomes: role of the LINE-encoded endonuclease.BMC genomics · 2011Article
- Bioinformatic analysis of Entamoeba histolytica SINE1 elements.BMC genomics · 2010Article
- Molecular methods for diagnosis of Entamoeba histolytica in a clinical setting: an overview.Experimental parasitology · 2007Review
- Transcriptional silencing of multiple genes in trophozoites of Entamoeba histolytica.PLoS pathogens · 2006Article
- Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species: potential implications for amebic pathogenesis.Infection and immunity · 2006Article
- Involvement of a short interspersed element in epigenetic transcriptional silencing of the amoebapore gene in Entamoeba histolytica.Eukaryotic cell · 2005Article
- Species- and strain-specific probes derived from repetitive DNA for distinguishing Entamoeba histolytica and Entamoeba dispar.Experimental parasitology · 2005Article
- Entamoeba histolytica and Entamoeba dispar utilize externalized phosphatidylserine for recognition and phagocytosis of erythrocytes.Infection and immunity · 2005Article
- Comparative genomic hybridizations of Entamoeba strains reveal unique genetic fingerprints that correlate with virulence.Eukaryotic cell · 2005Article
- An Entamoeba histolytica LINE/SINE pair inserts at common target sites cleaved by the restriction enzyme-like LINE-encoded endonuclease.Eukaryotic cell · 2004Article
- Characterization of cytosine methylated regions and 5-cytosine DNA methyltransferase (Ehmeth) in the protozoan parasite Entamoeba histolytica.Nucleic acids research · 2004Article
- Stress-dependent expression of a polymorphic, charged antigen in the protozoan parasite Entamoeba histolytica.Infection and immunity · 2003Article
- Evolutionary genomics of Entamoeba.Research in microbiologyReview
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3 authors at 1 institution in 1 country.
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Abstract
While comparing gene expression in the pathogenic organism Entamoeba histolytica and the closely related but nonpathogenic species Entamoeba dispar, we discovered that the E. histolytica abundant polyadenylated transcript 2 (ehapt2) and corresponding genomic copies are absent in E. dispar. Although polyadenylated, ehapt2 does not contain any overt open reading frame. Southern blot and sequence analyses revealed that about 500 copies of ehapt2 genomic elements were present in each cell and that the copies were distributed throughout the ameba genome. The various ehapt2 elements are regularly located in the vicinity of protein-encoding genes, downstream of pyrimidine-rich sequence stretches (40 to 125 bp; CT content, 79.2 to 85.5%), and are flanked by duplicated target sites of variable length. Target site duplications were obviously generated during integration of ehapt2 into the E. histolytica genome as one copy of the flanking repeat and the complete ehapt2 element are specifically absent in orthologous E. dispar genomic sequences. ehapt2 shares 3' sequences with EhRLE, a recently identified non-long-terminal-repeat (non-LTR) retrotransposon-like element of E. histolytica, which contains a conceptual open reading frame for reverse transcriptase. Thus, ehapt2 has all of the properties of nonautonomous non-LTR retrotransposons. A comparison of various E. histolytica isolates suggested that transposition of ehapt2 takes place at a very low frequency as the genomic localization of ehapt2 elements was found to be well conserved. A mobile element such as ehapt2 could be a suitable mechanism to explain the infrequent and late transition of E. histolytica from a harmless gut commensal to an invasive pathogen.
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