ArticleInfection and immunity2002
Enhanced immunogenicity of a genetic chimeric protein consisting of two virulence antigens of Streptococcus mutans and protection against infection.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 57 citations in OpenAlex.
- Assessing the Efficacy and Immunogenicity of Anticaries Vaccine-A Systematic Review and Meta-Analysis.Immunity, inflammation and disease · 2025Pooled it
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- An optimized secretory expression system and immunogenicity evaluation for glycosylated gp90 of avian reticuloendotheliosis virus.Veterinary research · 2020Article
- The Multifaceted Nature of Streptococcal Antigen I/II Proteins in Colonization and Disease Pathogenesis.Frontiers in microbiology · 2020Review
- Glycosyltransferase-Mediated Biofilm Matrix Dynamics and Virulence of Streptococcus mutans.Applied and environmental microbiology · 2019Article
- Self-assembling anticaries mucosal vaccine containing ferritin cage nanostructure and glucan-binding region of S. mutans glucosyltransferase effectively prevents caries formation in rodents.Human vaccines & immunotherapeutics · 2017Article
- Alterations in immunodominance of Streptococcus mutans AgI/II: lessons learned from immunomodulatory antibodies.Vaccine · 2014Article
- Comparing the cariogenic species Streptococcus sobrinus and S. mutans on whole genome level.Journal of oral microbiology · 2014Article
- Roles of salivary components in Streptococcus mutans colonization in a new animal model using NOD/SCID.e2f1-/- mice.PloS one · 2012Article
- Novel epitopic region of glucosyltransferase B from Streptococcus mutans.Clinical and vaccine immunology : CVI · 2011Article
- Contribution of a Streptococcus mutans antigen expressed by a Salmonella vector vaccine in dendritic cell activation.Infection and immunity · 2011Article
- Article
- A new small molecule specifically inhibits the cariogenic bacterium Streptococcus mutans in multispecies biofilms.Antimicrobial agents and chemotherapy · 2011Article
- Induction of neutralizing antibodies in mice immunized with an amino-terminal polypeptide of Streptococcus mutans P1 protein produced by a recombinant Bacillus subtilis strain.FEMS immunology and medical microbiology · 2010Article
- Good Manufacturing Practices production and analysis of a DNA vaccine against dental caries.Acta pharmacologica Sinica · 2009Article
- Beneficial immunomodulation by Streptococcus mutans anti-P1 monoclonal antibodies is Fc independent and correlates with increased exposure of a relevant target epitope.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Bactericidal antibody responses induced by meningococcal recombinant chimeric factor H-binding protein vaccines.Infection and immunity · 2008Article
- The scientific and public-health imperative for a vaccine against dental caries.Nature reviews. Immunology · 2006Review
- Immunological and protective effects of diepitopic subunit dental caries vaccines.Infection and immunity · 2005Article
- Redirecting the humoral immune response against Streptococcus mutans antigen P1 with monoclonal antibodies.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The saliva-binding region (SBR) of the cell surface antigen I/II (AgI/II) and the glucan-binding region (GLU) of the glucosyltransferase enzyme of Streptococcus mutans have been implicated in the initial adherence of S. mutans to saliva-coated tooth surfaces and the subsequent sucrose-dependent accumulation of S. mutans, respectively. Here, we describe the construction and characterization of a genetic chimeric protein consisting of the two virulence determinants SBR and GLU (SBR-GLU). The effectiveness of this construct in inducing mucosal and systemic immune responses to each virulence determinant following intranasal immunization was compared to that of each antigen alone or an equal mixture of SBR and GLU (SBR+GLU) in a mouse model. Furthermore, the ability of antibodies induced to SBR-GLU to protect against S. mutans infection was also investigated. Immunization of mice with the chimeric protein SBR-GLU resulted in significantly enhanced (P < 0.001) levels of serum immunoglobulin G (IgG) anti-SBR antibody activity compared to those in the SBR and SBR+GLU groups. The SBR-GLU-immunized mice also demonstrated a significant (P < 0.05) increase in salivary and vaginal IgA antibody responses to SBR and GLU. Analysis of the serum IgG subclass responses to SBR in mice immunized with SBR alone indicated a mixed IgG1 and IgG2a response. A preferential IgG1 response compared to an IgG2a anti-GLU response was induced in mice immunized with GLU alone. Similarly, a preferential IgG1 response was also induced to SBR when GLU was present in either a mixed or conjugated form. Finally, a significant reduction (P < 0.05) in S. mutans colonization was observed only in mice immunized with the SBR-GLU chimeric protein. Taken together, our results indicate that the chimeric protein SBR-GLU significantly enhanced mucosal immune responses to SBR and GLU and systemic immune responses to SBR. The ability of SBR-GLU to induce responses effective in protection against colonization of S. mutans suggests its potential as a vaccine antigen for dental caries.
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