ArticleThe Journal of biological chemistry2003
Trafficking of TrkA-green fluorescent protein chimerae during nerve growth factor-induced differentiation.
Article in The Journal of biological chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- Photosensitive tyrosine analogues unravel site-dependent phosphorylation in TrkA initiated MAPK/ERK signaling.Communications biology · 2020Article
- MYO9A deficiency in motor neurons is associated with reduced neuromuscular agrin secretion.Human molecular genetics · 2018Article
- Nerve Growth Factor Signaling from Membrane Microdomains to the Nucleus: Differential Regulation by Caveolins.International journal of molecular sciences · 2017Article
- The dynamics of trkA expression in the bovine ovary are associated with a luteotrophic effect of ovulation-inducing factor/nerve growth factor (OIF/NGF).Reproductive biology and endocrinology : RB&E · 2016Article
- Precursor and mature NGF live tracking: one versus many at a time in the axons.Scientific reports · 2016Article
- Ligand-induced dynamics of neurotrophin receptors investigated by single-molecule imaging approaches.International journal of molecular sciences · 2015Review
- Neurite outgrowth induced by NGF or L1CAM via activation of the TrkA receptor is sustained also by the exocytosis of enlargeosomes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Regulation of neurotrophin receptor (Trk) signaling: suppressor of cytokine signaling 2 (SOCS2) is a new player.Frontiers in molecular neuroscience · 2014Review
- Defective axonal transport of Rab7 GTPase results in dysregulated trophic signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Nanoparticle-mediated signaling endosome localization regulates growth cone motility and neurite growth.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Expressions of Axl and Tyro-3 receptors are under regulation of nerve growth factor and are involved in differentiation of PC12 cells.Neuroscience bulletin · 2011Article
- Heat shock protein 90 inhibition depletes TrkA levels and signaling in human acute leukemia cells.Molecular cancer therapeutics · 2010Article
- AMP-activated protein kinase supports the NGF-induced viability of human HeLa cells to glucose starvation.Molecular biology reports · 2010Article
- Axonal targeting of Trk receptors via transcytosis regulates sensitivity to neurotrophin responses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Interaction of Mint2 with TrkA is involved in regulation of nerve growth factor-induced neurite outgrowth.The Journal of biological chemistry · 2009Article
- The coming of age of axonal neurotrophin signaling endosomes.Journal of proteomics · 2009Review
- A neuron-specific cytoplasmic dynein isoform preferentially transports TrkB signaling endosomes.The Journal of cell biology · 2008Article
- Nerve growth factor involvement in liver cirrhosis and hepatocellular carcinoma.World journal of gastroenterology · 2007Article
- Nerve growth factor stimulates the concentration of TrkA within lipid rafts and extracellular signal-regulated kinase activation through c-Cbl-associated protein.Molecular and cellular biology · 2007Article
- A novel endocytic recycling signal distinguishes biological responses of Trk neurotrophin receptors.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
Abstract
A chimera of the nerve growth factor (NGF) receptor, TrkA, and green fluorescent protein (GFP) was engineered by expressing GFP in phase with the carboxyl terminus of TrkA. TrkA-GFP becomes phosphorylated on tyrosine residues in response to NGF and is capable of initiating signaling cascades leading to prolonged MAPK activation and differentiation in PC12 nnr5 cells. TrkA constructs, progressively truncated in the carboxyl-terminal domain, were prepared as GFP chimerae in order to identify which part of the receptor intracellular domain is involved in its trafficking. Immunofluorescence observations show that TrkA-GFP is found mainly in cell surface membrane ruffles and in endosomes. Biochemical analysis indicated that the cytoplasmic domain of TrkA is not necessary for correct maturation and cell surface translocation of the receptor. An antibody against the extracellular domain of TrkA (RTA) was used as ligand to stimulate internalization and phosphorylation of TrkA. Co-localization studies with anti-phosphorylated TrkA antibodies support a role for such complexes in the propagation of signaling from the cell surface, resulting in the activation of TrkA in areas of the endosome devoid of receptor-ligand complexes. Confocal time-lapse analysis reveals that the TrkA-GFP chimera shows highly dynamic trafficking between the cell surface and internal locations. TrkA-positive vesicles were estimated to move 0.46 +/- 0.09 microm/s anterograde and 0.48 +/- 0.07 microm/s retrograde. This approach and the fidelity of the biochemical properties of the TrkA-GFP demonstrate that real-time visualization of trafficking of tyrosine kinase receptors in the presence or absence of the ligand is feasible.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.