ArticleThe Biochemical journal2003
Cloning and kinetic characterization of Arabidopsis thaliana solanesyl diphosphate synthase.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 65 citations in OpenAlex.
- Farnesol and geranylgeraniol in plant reproduction: insights from Arabidopsis and beyond.Journal of experimental botany · 2026Review
- Mysterious giants in the world of lipids: long linear isoprenoid functions in plant physiology and reproduction.Journal of experimental botany · 2026Review
- Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis.Antioxidants (Basel, Switzerland) · 2023Review
- Comparative de novo transcriptome analysis identifies salinity stress responsive genes and metabolic pathways in sugarcane and its wild relative Erianthus arundinaceus [Retzius] Jeswiet.Scientific reports · 2021Article
- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP) for Metabolome and Proteome Analyses.Plant physiology · 2020Article
- Functional Gene Network of Prenyltransferases inMolecules (Basel, Switzerland) · 2019Review
- Natural rubber biosynthesis in plants, the rubber transferase complex, and metabolic engineering progress and prospects.Plant biotechnology journal · 2019Review
- Enzyme Fusion Removes Competition for Geranylgeranyl Diphosphate in Carotenogenesis.Plant physiology · 2019Article
- Functional Analysis of Polyprenyl Diphosphate Synthase Genes Involved in Plastoquinone and Ubiquinone Biosynthesis inFrontiers in plant science · 2019Article
- Vitamin E Biosynthesis and Its Regulation in Plants.Antioxidants (Basel, Switzerland) · 2017Review
- Metabolic Origins and Transport of Vitamin E Biosynthetic Precursors.Frontiers in plant science · 2017Review
- Conserved Function of Fibrillin5 in the Plastoquinone-9 Biosynthetic Pathway in Arabidopsis and Rice.Frontiers in plant science · 2017Article
- Suppressing Farnesyl Diphosphate Synthase Alters Chloroplast Development and Triggers Sterol-Dependent Induction of Jasmonate- and Fe-Related Responses.Plant physiology · 2016Article
- Plastoquinone and Ubiquinone in Plants: Biosynthesis, Physiological Function and Metabolic Engineering.Frontiers in plant science · 2016Review
- Fibrillin 5 Is Essential for Plastoquinone-9 Biosynthesis by Binding to Solanesyl Diphosphate Synthases in Arabidopsis.The Plant cell · 2015Article
- Determination of residues responsible for substrate and product specificity of Solanum habrochaites short-chain cis-prenyltransferases.Plant physiology · 2014Article
- Functional modeling identifies paralogous solanesyl-diphosphate synthases that assemble the side chain of plastoquinone-9 in plastids.The Journal of biological chemistry · 2013Article
- Article
- A transcriptional analysis of carotenoid, chlorophyll and plastidial isoprenoid biosynthesis genes during development and osmotic stress responses in Arabidopsis thaliana.BMC systems biology · 2011Article
- Structure and mechanism of an Arabidopsis medium/long-chain-length prenyl pyrophosphate synthase.Plant physiology · 2011Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
trans -Long-chain prenyl diphosphate synthases catalyse the sequential condensation of isopentenyl diphosphate (C(5)) units with allylic diphosphate to produce the C(30)-C(50) prenyl diphosphates, which are precursors of the side chains of prenylquinones. Based on the relationship between product specificity and the region around the first aspartate-rich motif in trans -prenyl diphosphate synthases characterized so far, we have isolated the cDNA for a member of trans -long-chain prenyl diphosphate synthases from Arabidopsis thaliana. The cDNA was heterologously expressed in Escherichia coli, and the recombinant His(6)-tagged protein was purified and characterized. Product analysis revealed that the cDNA encodes solanesyl diphosphate (C(45)) synthase (At-SPS). At-SPS utilized farnesyl diphosphate (FPP; C(15)) and geranylgeranyl diphosphate (GGPP; C(20)), but did not accept either the C(5) or the C(10) allylic diphosphate as a primer substrate. The Michaelis constants for FPP and GGPP were 5.73 microM and 1.61 microM respectively. We also performed an analysis of the side chains of prenylquinones extracted from the A. thaliana plant, and showed that its major prenylquinones, i.e. plastoquinone and ubiquinone, contain the C(45) prenyl moiety. This suggests that At-SPS might be devoted to the biosynthesis of either or both of the prenylquinone side chains. This is the first established trans -long-chain prenyl diphosphate synthase from a multicellular organism.
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