ArticleTransgenic research2002
A chimeric ecdysone receptor facilitates methoxyfenozide-dependent restoration of male fertility in ms45 maize.
Article in Transgenic research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Design and evolution of the tetracycline repressor into sulfonylurea herbicide-responsive gene switches for field crops.Nature communications · 2026Article
- Maize Anther Development Involves Translated Open Reading Frames From 3' Untranslated Regions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Three strategies of transgenic manipulation for crop improvement.Frontiers in plant science · 2022Review
- The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins.Plant molecular biology · 2016Article
- Development of a bipartite ecdysone-responsive gene switch for the oomycete Phytophthora infestans and its use to manipulate transcription during axenic culture and plant infection.Molecular plant pathology · 2015Article
- Transcriptional silencing of heterologous anther promoters in maize: a genetic method to replace detasseling for seed production.Plant reproduction · 2014Article
- An improved chemically inducible gene switch that functions in the monocotyledonous plant sugar cane.Plant molecular biology · 2014Article
- Designer promoter: an artwork of cis engineering.Plant molecular biology · 2011Review
- Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds.Transgenic research · 2010Article
- Promoter diversity in multigene transformation.Plant molecular biology · 2010Review
- Chemically inducible expression of the PHB biosynthetic pathway in Arabidopsis.Transgenic research · 2007Article
- Development of a tightly regulated and highly inducible ecdysone receptor gene switch for plants through the use of retinoid X receptor chimeras.Transgenic research · 2007Article
- Practical uses for ecdysteroids in mammals including humans: an update.Journal of insect science (Online) · 2003Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mutation in the maize Ms45 gene results in abortion of microspore development and a male-sterile phenotype. MS45 protein has been localized to the tapetum and maximally expressed in anthers at the early vacuolate stage of microspore development. Molecular complementation analysis determined that a transformed copy of the gene fully restored fertility to ms45 maize. In this report, using phenotypic complementation as an assay, chimeric transcriptional activators were expressed to regulate a gal:MS45 gene and test the ability of a multi-component system to restore male fertility. A high frequency of phenotypic complementation was observed when either C1-GAL4 or VP16-GAL4 activators were transcribed by promoters that expressed at a stage of anther development that precedes the early vacuolate stage of microsporogenesis. For the conditional regulation of male fertility, these transcriptional activators were modified by the addition of regions that include the ligand-binding domain from the European corn borer ecdysone receptor to generate the nuclear receptors C1-GAL4-EcR (CGEcR) and VP16-GAL4-EcR (VGEcR). These chimeric receptors were introduced with the gal:MS45 gene into ms45 maize, and in the absence of ligand, these plants were male sterile. In contrast, application of the ecdysone agonist, methoxyfenozide, to plants containing either a constitutive (Ubiquitin1) or anther-specific (maize 5126) VGEcR resulted in the restoration of fertility to ms45 plants grown in either the greenhouse or in the field.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.