ArticleNucleic acids research2002
A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Bacterial Pathogen Infection Triggers Magic Spot Nucleotide Signaling inJournal of the American Chemical Society · 2023Article
- Identification of a RelA/SpoT Homolog and Its Possible Role in the Accumulation of Astaxanthin inFrontiers in plant science · 2022Article
- The Role of RelA and SpoT on ppGpp Production, Stress Response, Growth Regulation, and Pathogenicity in Xanthomonas campestris pv.Microbiology spectrum · 2021Article
- ppGpp functions as an alarmone in metazoa.Communications biology · 2020Article
- Functional Characterization of a Small Alarmone Hydrolase inFrontiers in microbiology · 2018Article
- Review
- Identification and Functional Characterization of Small Alarmone Synthetases inFrontiers in microbiology · 2017Article
- An Ancient Bacterial Signaling Pathway Regulates Chloroplast Function to Influence Growth and Development in Arabidopsis.The Plant cell · 2016Article
- Photosynthetic gene expression in higher plants.Photosynthesis research · 2013Review
- Review
- The magic spot ppGpp influences in vitro the molecular and functional properties of the elongation factor 1α from the archaeon Sulfolobus solfataricus.Extremophiles : life under extreme conditions · 2012Article
- ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro.Plant molecular biology · 2012Article
- Role of the all1549 (ana-rsh) gene, a relA/spoT homolog, of the Cyanobacterium Anabaena sp. PCC7120.Current microbiology · 2011Article
- A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses.Nature structural & molecular biology · 2010Article
- Possible targets of "magic spots" in plant signalling.Plant signaling & behavior · 2008Article
- Identification of the bacterial alarmone guanosine 5'-diphosphate 3'-diphosphate (ppGpp) in plants.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Null mutation of HvrA compensates for loss of an essential relA/spoT-like gene in Rhodobacter capsulatus.Journal of bacteriology · 2004Article
- Guanosine tetra- and pentaphosphate synthase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline.Nucleic acids research · 2004Article
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A gene encoding a putative guanosine 3',5'-bispyrophosphate (ppGpp) synthase-degradase, designated Cr-RSH, was identified in the unicellular photosynthetic eukaryote Chlamydomonas reinhardtii. The encoded Cr-RSH protein possesses a putative chloroplast-targeting signal at its NH2-terminus, and translocation of Cr-RSH into chloroplasts isolated from C.reinhardtii was demonstrated in vitro. The predicted mature region of Cr-RSH exhibits marked similarity to eubacterial members of the RelA-SpoT family of proteins. Expression of an NH2-terminal portion of Cr-RSH containing the putative ppGpp synthase domain in a relA, spoT double mutant of Escherichia coli complemented the growth deficits of the mutant cells. Chromatographic analysis of 32P-labeled cellular mononucleotides also revealed that expression of Cr-RSH in the mutant bacterial cells resulted in the synthesis of ppGpp. SpoT, which catalyzes (p)ppGpp degradation, is dispensable in E.coli only if cells also lack RelA, which possesses (p)ppGpp synthase activity. The complementation analysis thus indicated that Cr-RSH possesses both ppGpp synthase and degradase activities. These results represent the first demonstration of ppGpp synthase-degradase activities in a eukaryotic organism, and they suggest that eubacterial stringent control mediated by ppGpp has been conserved during evolution of the chloroplast from a photosynthetic bacterial symbiont.
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