ArticleNucleic acids research2002
Role of tumor suppressor p53 domains in selective binding to supercoiled DNA.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 60 citations in OpenAlex.
- Professor Emil Paleček: seven decades with electrodes and biomolecules at the Institute of Biophysics of the CAS.European biophysics journal : EBJ · 2025Article
- A novel germline mutation of TP53 with breast cancer diagnosed as Li-Fraumeni syndrome.Surgical case reports · 2022Article
- 1,3-Diketone-Modified Nucleotides and DNA for Cross-Linking with Arginine-Containing Peptides and Proteins.Angewandte Chemie (International ed. in English) · 2021Article
- Metabolic Pathways Enhancement Confers Poor Prognosis in p53 Exon Mutant Hepatocellular Carcinoma.Cancer informatics · 2020Article
- Squaramate-Modified Nucleotides and DNA for Specific Cross-Linking with Lysine-Containing Peptides and Proteins.Angewandte Chemie (International ed. in English) · 2019Article
- p53 Binds Preferentially to Non-B DNA Structures Formed by the Pyrimidine-Rich Strands of GAA·TTC Trinucleotide Repeats Associated with Friedreich's Ataxia.Molecules (Basel, Switzerland) · 2019Article
- Investigating the Influence of Magnesium Ions on p53-DNA Binding Using Atomic Force Microscopy.International journal of molecular sciences · 2017Article
- Wild-type p53 binds to MYC promoter G-quadruplex.Bioscience reports · 2016Article
- Solvatochromic fluorene-linked nucleoside and DNA as color-changing fluorescent probes for sensing interactions.Chemical science · 2016Article
- p53 Specifically Binds Triplex DNA In Vitro and in Cells.PloS one · 2016Article
- Differential salt-induced dissociation of the p53 protein complexes with circular and linear plasmid DNA substrates suggest involvement of a sliding mechanism.International journal of molecular sciences · 2015Article
- Azidophenyl as a click-transformable redox label of DNA suitable for electrochemical detection of DNA-protein interactions.Chemical science · 2015Article
- Preferential binding of hot spot mutant p53 proteins to supercoiled DNA in vitro and in cells.PloS one · 2013Article
- Cruciform structures are a common DNA feature important for regulating biological processes.BMC molecular biology · 2011Review
- Improving the assessment of the outcome of nonsynonymous SNVs with a consensus deleteriousness score, Condel.American journal of human genetics · 2011Article
- The expanding universe of p53 targets.Nature reviews. Cancer · 2009Review
- Modulation of gene expression in U251 glioblastoma cells by binding of mutant p53 R273H to intronic and intergenic sequences.Nucleic acids research · 2009Article
- Functional four-base A/T gap core sequence CATTAG of P53 response elements specifically bound tetrameric P53 differently than two-base A/T gap core sequence CATG bound both dimeric and tetrameric P53.Nucleic acids research · 2009Article
- Change of the protein p53 electrochemical signal according to its structural form - quick and sensitive distinguishing of native, denatured, and aggregated form of the "guardian of the genome".The protein journal · 2006Article
- Wild-type p53 stimulates homologous recombination upon sequence-specific binding to the ribosomal gene cluster repeat.Oncogene · 2005Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We showed previously that bacterially expressed full-length human wild-type p53b(1-393) binds selectively to supercoiled (sc)DNA in sc/linear DNA competition experiments, a process we termed supercoil-selective (SCS) binding. Using p53 deletion mutants and pBluescript scDNA (lacking the p53 recognition sequence) at native superhelix density we demonstrate here that the p53 C-terminal domain (amino acids 347-382) and a p53 oligomeric state are important for SCS binding. Monomeric p53(361-393) protein (lacking the p53 tetramerization domain, amino acids 325-356) did not exhibit SCS binding while both dimeric mutant p53(319- 393)L344A and fusion protein GCN4-p53(347-393) were effective in SCS binding. Supershifting of p53(320-393)-scDNA complexes with monoclonal antibodies revealed that the amino acid region 375-378, constituting the epitope of the Bp53-10.1 antibody, plays a role in binding of the p53(320-393) protein to scDNA. Using electron microscopy we observed p53-scDNA nucleoprotein filaments produced by all the C-terminal proteins that displayed SCS binding in the gel electrophoresis experiments; no filaments formed with the monomeric p53(361- 393) protein. We propose a model according to which two DNA duplexes are compacted into p53-scDNA filaments and discuss a role for filament formation in recombination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.