ArticleProceedings of the National Academy of Sciences of the United States of America2002
SWI/SNF-dependent long-range remodeling of yeast HIS3 chromatin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 37 citations in OpenAlex.
- Context-dependent interplay of Swi-Snf and Tup1-Cyc8 influences chromatin and transcription at antagonistically regulated genes.Nature communications · 2026Article
- Modeling a disease-correlated tubulin mutation in budding yeast reveals insight into MAP-mediated dynein function.Molecular biology of the cell · 2021Article
- Interplay among ATP-Dependent Chromatin Remodelers Determines Chromatin Organisation in Yeast.Biology · 2020Review
- Coming to terms with chromatin structure.Chromosoma · 2016Review
- The importance of being supercoiled: how DNA mechanics regulate dynamic processes.Biochimica et biophysica acta · 2012Review
- Activation-induced disruption of nucleosome position clusters on the coding regions of Gcn4-dependent genes extends into neighbouring genes.Nucleic acids research · 2011Article
- A polar barrier to transcription can be circumvented by remodeler-induced nucleosome translocation.Nucleic acids research · 2011Article
- Spt10 and Swi4 control the timing of histone H2A/H2B gene activation in budding yeast.Molecular and cellular biology · 2011Article
- Nucleosome positioning, nucleosome spacing and the nucleosome code.Journal of biomolecular structure & dynamics · 2010Article
- Activation and repression domains within the promoter of the rat cathepsin L gene orchestrate sertoli cell-specific and stage-specific gene transcription in transgenic mice.Biology of reproduction · 2009Article
- Cooperative regulation of ADE3 transcription by Gcn4p and Bas1p in Saccharomyces cerevisiae.Eukaryotic cell · 2009Article
- SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter.Molecular and cellular biology · 2009Article
- Spn1 regulates the recruitment of Spt6 and the Swi/Snf complex during transcriptional activation by RNA polymerase II.Molecular and cellular biology · 2008Article
- Article
- Exposing the core promoter is sufficient to activate transcription and alter coactivator requirement at RNR3.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Contribution of the serine 129 of histone H2A to chromatin structure.Molecular and cellular biology · 2007Article
- Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A.Virology · 2007Article
- Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi.Virology · 2007Article
- Tup1-Ssn6 and Swi-Snf remodelling activities influence long-range chromatin organization upstream of the yeast SUC2 gene.Nucleic acids research · 2007Article
- Activation of Saccharomyces cerevisiae HIS3 results in Gcn4p-dependent, SWI/SNF-dependent mobilization of nucleosomes over the entire gene.Molecular and cellular biology · 2006Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Current models for the role of the SWISNF chromatin remodeling complex in gene regulation are focused on promoters, where the most obvious changes in chromatin structure occur. Here we present evidence that the SWISNF complex is involved in the remodeling of the chromatin structure of an entire gene in vivo. We compared the native chromatin structures of a small yeast plasmid containing the HIS3 gene purified from uninduced and induced cells. Relative to uninduced chromatin, induced chromatin displayed a large reduction in negative supercoiling, a large reduction in sedimentation rate, and increased accessibility to restriction enzymes with sites located both near and far from the HIS3 promoter. These observations indicate that the entire plasmid was remodeled as a result of induction. Loss of supercoiling required the presence of the SWISNF remodeling complex and the activator Gcn4p in vivo. The TATA boxes were not required, suggesting that remodeling was not the result of transcription. The induction-dependent loss of negative supercoiling was not apparent in cells, indicating that the supercoils were lost preferentially from induced chromatin during purification. Thus, induced HIS3 chromatin has a highly labile structure that is revealed as a result of purification. It is concluded that induction of HIS3 creates a domain of labile chromatin structure that extends far beyond the promoter to include the entire gene. We propose that the SWISNF complex is recruited to the HIS3 promoter by Gcn4p and then directs remodeling of a chromatin domain, with important implications for transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.