ArticleMolecular biology of the cell2002
Cofilin, but not profilin, is required for myosin-I-induced actin polymerization and the endocytic uptake in yeast.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Apical clathrin-coated pits control the location, timing, and scale of microvillar growth.Cell reports · 2025Article
- Synthesis and Development of a Novel First-in-Class Cofilin Inhibitor for Neuroinflammation in Hemorrhagic Brain Injury.ACS chemical neuroscience · 2022Article
- Article
- An Engineered Minimal WASP-Myosin Fusion Protein Reveals Essential Functions for Endocytosis.Developmental cell · 2015Article
- Profilin is required for Ca2+ homeostasis and Ca2+-modulated bud formation in yeast.Molecular genetics and genomics : MGG · 2013Article
- A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis.PLoS biology · 2011Article
- Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.The EMBO journal · 2010Article
- The actin-binding protein capulet genetically interacts with the microtubule motor kinesin to maintain neuronal dendrite homeostasis.PloS one · 2008Article
- Cofilin recruitment and function during actin-mediated endocytosis dictated by actin nucleotide state.The Journal of cell biology · 2007Article
- Roles of type II myosin and a tropomyosin isoform in retrograde actin flow in budding yeast.The Journal of cell biology · 2006Article
- The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p.The Journal of cell biology · 2006Article
- Functional characterization of myosin I tail regions in Candida albicans.Eukaryotic cell · 2004Article
- Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance.The Journal of cell biology · 2004Article
- Saccharomyces cerevisiae Bzz1p is implicated with type I myosins in actin patch polarization and is able to recruit actin-polymerizing machinery in vitro.Molecular and cellular biology · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutations in the budding yeast myosins-I (MYO3 and MYO5) cause defects in the actin cytoskeleton and in the endocytic uptake. Robust evidence also indicates that these proteins induce Arp2/3-dependent actin polymerization. Consistently, we have recently demonstrated, using fluorescence microscopy, that Myo5p is able to induce cytosol-dependent actin polymerization on the surface of Sepharose beads. Strikingly, we now observed that, at short incubation times, Myo5p induced the formation of actin foci that resembled the yeast cortical actin patches, a plasma membrane-associated structure that might be involved in the endocytic uptake. Analysis of the machinery required for the formation of the Myo5p-induced actin patches in vitro demonstrated that the Arp2/3 complex was necessary but not sufficient in the assay. In addition, we found that cofilin was directly involved in the process. Strikingly though, the cofilin requirement seemed to be independent of its ability to disassemble actin filaments and profilin, a protein that closely cooperates with cofilin to maintain a rapid actin filament turnover, was not needed in the assay. In agreement with these observations, we found that like the Arp2/3 complex and the myosins-I, cofilin was essential for the endocytic uptake in vivo, whereas profilin was dispensable.
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