ArticleMolecular biology of the cell2002
Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- Article
- Persistent DNA damage underlies tubular cell polyploidization and progression to chronic kidney disease in kidneys deficient in the DNA repair protein FAN1.Kidney international · 2022Article
- APC/CScientific reports · 2016Article
- p53 activates G₁ checkpoint following DNA damage by doxorubicin during transient mitotic arrest.Oncotarget · 2015Article
- CDC6 expression is regulated by lineage-specific transcription factor GATA1.Cell cycle (Georgetown, Tex.) · 2012Article
- Proposed megakaryocytic regulon of p53: the genes engaged to control cell cycle and apoptosis during megakaryocytic differentiation.Physiological genomics · 2012Article
- RUNX1-induced silencing of non-muscle myosin heavy chain IIB contributes to megakaryocyte polyploidization.Nature communications · 2012Article
- Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication.BMC cell biology · 2011Article
- Geminin deletion from hematopoietic cells causes anemia and thrombocytosis in mice.The Journal of clinical investigation · 2010Article
- Endoreplication: polyploidy with purpose.Genes & development · 2009Review
- Effect of leptin on mouse trophoblast giant cells.Biology of reproduction · 2009Article
- Mechanisms to control rereplication and implications for cancer.Current opinion in cell biology · 2007Review
- Reverse genetic analysis of the yeast RSC chromatin remodeler reveals a role for RSC3 and SNF5 homolog 1 in ploidy maintenance.PLoS genetics · 2007Article
- p53-Dependent regulation of Cdc6 protein stability controls cellular proliferation.Molecular and cellular biology · 2005Article
- Anaphase-promoting complex-dependent proteolysis of cell cycle regulators and genomic instability of cancer cells.Oncogene · 2005Review
- Overexpression of the replication licensing regulators hCdt1 and hCdc6 characterizes a subset of non-small-cell lung carcinomas: synergistic effect with mutant p53 on tumor growth and chromosomal instability--evidence of E2F-1 transcriptional control over hCdt1.The American journal of pathology · 2004Article
- DNA replication licensing affects cell proliferation or endoreplication in a cell type-specific manner.The Plant cell · 2004Article
- Bovine papillomavirus replicative helicase E1 is a target of the ubiquitin ligase APC.Journal of virology · 2004Article
- A novel E2 box-GATA element modulates Cdc6 transcription during human cells polyploidization.Nucleic acids research · 2004Article
- Caspase-3-mediated cleavage of Cdc6 induces nuclear localization of p49-truncated Cdc6 and apoptosis.Molecular biology of the cell · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endomitosis is the process by which mammalian megakaryocytes become polyploid during terminal differentiation. As in other endoreplicating cells, cyclin-cdk complexes are distinctly regulated, probably to overcome the strict mechanisms that prevent rereplication in most somatic cells. We have asked whether key factors involved in the assembly and licensing of replication origins are equally regulated during endomitosis. Cdc6, cdt1, and geminin expression was analyzed during differentiation of two human megakaryoblastic cell lines, HEL and K562, which respectively do and do not establish endoreplication cycles. Geminin was downregulated, whereas cdt1 levels were maintained upon differentiation of both cell lines, independently of whether cells entered extra S-phases. In contrast, cdc6 was present and remained nuclear only in differentiated endoreplicating cells. Interestingly, cdc6 protein expression was reestablished in K562 cells that underwent endomitosis after transient or stable cyclin E overexpression. The high levels of cyclin E reached in these cells appeared to influence the stabilization of cdc6 protein rather than its RNA transcription rate. Finally, cdc6 overexpression drove HEL cells into endoreplication cycles in the absence of differentiation stimuli. Our results show that both cdt1 and cdc6 are differentially regulated during megakaryocytic differentiation and suggest an active role of cdc6 in endomitosis.
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