ArticleMolecular biology of the cell2002
Identification of a novel type of cGMP phosphodiesterase that is defective in the chemotactic stmF mutants.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Diverse Roles of the Multiple Phosphodiesterases in the Regulation of Cyclic Nucleotide Signaling inCells · 2025Review
- Analysis of cGMP Signaling in Dictyostelium.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Forty-five years of cGMP research inMolecular biology of the cell · 2021Review
- A cyanobacterial light activated adenylyl cyclase partially restores development of a Dictyostelium discoideum, adenylyl cyclase a null mutant.Journal of biotechnology · 2014Article
- The prokaryote messenger c-di-GMP triggers stalk cell differentiation in Dictyostelium.Nature · 2012Article
- Functional dissection of adenylate cyclase R, an inducer of spore encapsulation.The Journal of biological chemistry · 2010Article
- Domain mapping on the human metastasis regulator protein h-Prune reveals a C-terminal dimerization domain.The Biochemical journal · 2007Article
- Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP.The Biochemical journal · 2007Article
- Release of Ca2+ from the endoplasmic reticulum contributes to Ca2+ signaling in Dictyostelium discoideum.Eukaryotic cell · 2005Article
- cAMP controls cytosolic Ca2+ levels in Dictyostelium discoideum.BMC cell biology · 2005Article
- Dictyostelium stress-activated protein kinase alpha, a novel stress-activated mitogen-activated protein kinase kinase kinase-like kinase, is important for the proper regulation of the cytoskeleton.Molecular biology of the cell · 2003Article
- Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium.Molecular biology of the cell · 2002Article
- A model for cGMP signal transduction in Dictyostelium in perspective of 25 years of cGMP research.Journal of muscle research and cell motility · 2002Review
- cAMP signaling in Dictyostelium. Complexity of cAMP synthesis, degradation and detection.Journal of muscle research and cell motility · 2002Review
- Signaling pathways regulating Dictyostelium myosin II.Journal of muscle research and cell motility · 2002Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
StmF mutants are chemotactic mutants that are defective in a cGMP phosphodiesterase (PDE) activity. We identified a novel gene, PdeD, that harbors two cyclic nucleotide-binding domains and a metallo-beta-lactamase homology domain. Similar to stmF mutants, pdeD-null mutants displayed extensively streaming aggregates, prolonged elevation of cGMP levels after chemotactic stimulation, and reduced cGMP-PDE activity. PdeD transcripts were lacking in stmF mutant NP377, indicating that this mutant carries a PdeD lesion. Expression of a PdeD-YFP fusion protein in pdeD-null cells restored the normal cGMP response and showed that PdeD resides in the cytosol. When purified by immunoprecipitation, the PdeD-YFP fusion protein displayed cGMP-PDE activity, which was retained in a truncated construct that contained only the metallo-beta-lactamase domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.