ArticleMolecular biology of the cell2002
Stage-specific requirement of a mitogen-activated protein kinase by Trypanosoma brucei.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 62 citations in OpenAlex.
- Piplartine-Inspired 3,4,5-Trimethoxycinnamates: Trypanocidal, Mechanism of Action, and In Silico Evaluation.Molecules (Basel, Switzerland) · 2023Article
- The genomic basis of host and vector specificity in non-pathogenic trypanosomatids.Biology open · 2022Article
- An Alternative Strategy for Trypanosome Survival in the Mammalian Bloodstream Revealed through Genome and Transcriptome Analysis of the Ubiquitous Bovine Parasite Trypanosoma (Megatrypanum) theileri.Genome biology and evolution · 2017Article
- Extracellular-signal regulated kinase 8 of Trypanosoma brucei uniquely phosphorylates its proliferating cell nuclear antigen homolog and reveals exploitable properties.Cell cycle (Georgetown, Tex.) · 2016Article
- Quantitative Proteomics Uncovers Novel Factors Involved in Developmental Differentiation of Trypanosoma brucei.PLoS pathogens · 2016Article
- Transferrin: Endocytosis and Cell Signaling in Parasitic Protozoa.BioMed research international · 2015Review
- Quantitative proteomic and phosphoproteomic analysis of Trypanosoma cruzi amastigogenesis.Molecular & cellular proteomics : MCP · 2014Article
- Kinases as druggable targets in trypanosomatid protozoan parasites.Chemical reviews · 2014Review
- Distinct roles of a mitogen-activated protein kinase in cytokinesis between different life cycle forms of Trypanosoma brucei.Eukaryotic cell · 2014Article
- Hypothemycin, a fungal natural product, identifies therapeutic targets in Trypanosoma brucei [corrected].eLife · 2013Article
- Identification of essential and non-essential protein kinases by a fusion PCR method for efficient production of transgenic Trypanosoma brucei.Molecular and biochemical parasitology · 2013Article
- Regulation of Trypanosoma brucei Total and Polysomal mRNA during Development within Its Mammalian Host.PloS one · 2013Article
- Trypanosoma brucei Orc1 is essential for nuclear DNA replication and affects both VSG silencing and VSG switching.Molecular microbiology · 2013Article
- Third target of rapamycin complex negatively regulates development of quiescence in Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A phosphoproteomic approach towards the understanding of the role of TGF-β in Trypanosoma cruzi biology.PloS one · 2012Article
- Trypanosoma brucei: Two mitogen activated protein kinase kinases are dispensable for growth and virulence of the bloodstream form.Experimental parasitology · 2011Article
- Parasite mitogen-activated protein kinases as drug discovery targets to treat human protozoan pathogens.Journal of signal transduction · 2011Article
- The Trypanosoma brucei life cycle switch TbPTP1 is structurally conserved and dephosphorylates the nucleolar protein NOPP44/46.The Journal of biological chemistry · 2010Article
- Trypanosoma brucei: reduction of GPI-phospholipase C protein during differentiation is dependent on replication of newly transformed cells.Experimental parasitology · 2010Article
- Leishmania major MPK7 protein kinase activity inhibits intracellular growth of the pathogenic amastigote stage.Eukaryotic cell · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In cycling between the mammalian host and the tsetse fly vector, African trypanosomes undergo adaptive differentiation steps that are coupled to growth control. The signaling pathways underlying these cellular processes are largely unknown. Mitogen-activated protein kinases (MAPKs) are known mediators of growth and differentiation in other eukaryotic organisms. To establish the function of a MAPK homologue, TbMAPK2, in T. brucei, a null mutant was constructed. Bloodstream forms of a deltamapk2/deltamapk2 clone were able to grow normally and exhibited no detectable phenotype. When these cells were triggered to differentiate in vitro, however, they developed to the procyclic (fly midgut) form with delayed kinetics and subsequently underwent cell cycle arrest. Introduction of an ectopic copy of the TbMAPK2 gene into the null mutant restored its ability to differentiate and to divide. In contrast, a TbMAPK2 mutant, in which the T190 and Y192 residues of the activating phosphorylation site were replaced by A and F, was unable to restore the growth and differentiation phenotypes. Analysis of the DNA content and the nucleus/kinetoplast configuration of individual cells showed that the null mutant was arrested in all phases of the cell cycle and that 25-30% of the cells had failed to segregate their nucleus and kinetoplast correctly. This implies that cell cycle progression by the procyclic form depends on a constitutive stimulus exerted by the signaling cascade operating through TbMAPK2.
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Registered trials
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