ArticleThe Journal of cell biology2002
Hsc70 is required for endocytosis and clathrin function in Drosophila.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 127 citations in OpenAlex.
- Hsc70-4: An unanticipated mediator of dsRNA internalization inScience advances · 2025Article
- Dectin-1 stimulating β-glucans inhibit Chlamydia infections both in vitro and in vivo.Pathogens and disease · 2025Article
- Endocytosis of Wnt ligands from surrounding epithelial cells positions microtubule nucleation sites at dendrite branch points.PLoS biology · 2025Article
- Peripheral nerves directly mediate the transneuronal translocation of silver nanomaterials from the gut to central nervous system.Science advances · 2023Article
- Parkinson disease-associated Leucine-rich repeat kinase regulates UNC-104-dependent axonal transport of Arl8-positive vesicles iniScience · 2022Article
- Clearance of variant Creutzfeldt-Jakob disease prions in vivo by the Hsp70 disaggregase system.Brain : a journal of neurology · 2022Article
- Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila.Communications biology · 2022Article
- Drosophila melanogaster: a simple genetic model of kidney structure, function and disease.Nature reviews. Nephrology · 2022Review
- De novo endocytic clathrin coats develop curvature at early stages of their formation.Developmental cell · 2021Article
- A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience.G3 (Bethesda, Md.) · 2021Article
- A widespread family of heat-resistant obscure (Hero) proteins protect against protein instability and aggregation.PLoS biology · 2020Article
- Amyotrophic Lateral Sclerosis: Proteins, Proteostasis, Prions, and Promises.Frontiers in cellular neuroscience · 2020Article
- Mechanism and Complex Roles of HSC70 in Viral Infections.Frontiers in microbiology · 2020Review
- HSPA8/HSC70 in Immune Disorders: A Molecular Rheostat that Adjusts Chaperone-Mediated Autophagy Substrates.Cells · 2019Review
- RNA-Seq analysis of Polyrhachis vicina Roger and insights into the heat shock protein 90 and 70 families.Cell stress & chaperones · 2019Article
- Reconfiguration of a Multi-oscillator Network by Light in the Drosophila Circadian Clock.Current biology : CB · 2018Article
- Molecular Chaperone Hsp70 and Its Constitutively Active Form Hsc70 Play an Indispensable Role During Eye Development of Drosophila melanogaster.Molecular neurobiology · 2018Article
- Post-transcriptional Inhibition of Hsc70-4/HSPA8 Expression Leads to Synaptic Vesicle Cycling Defects in Multiple Models of ALS.Cell reports · 2017Article
- Dissecting the Role of the Extracellular Matrix in Heart Disease: Lessons from the Drosophila Genetic Model.Veterinary sciences · 2017Review
- Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
By screening for Drosophila mutants exhibiting aberrant bride of sevenless (Boss) staining patterns on eye imaginal disc epithelia, we have recovered a point mutation in Hsc70-4, the closest homologue to bovine clathrin uncoating ATPase. Although the mutant allele was lethal, analysis of mutant clones generated by FLP/FRT recombination demonstrated that the Sevenless-mediated internalization of Boss was blocked in mutant Hsc70-4 eye disc epithelial cells. Endocytosis of other probes was also greatly inhibited in larval Garland cells. Immunostaining and EM analysis of the mutant cells revealed disruptions in the organization of endosomal/lysosomal compartments, including a substantial reduction in the number of clathrin-coated structures in Garland cells. The Hsc70-4 mutation also interacted genetically with a dominant-negative mutant of dynamin, a gene required for the budding of clathrin-coated vesicles (CCVs). Consistent with these phenotypes, recombinant mutant Hsc70 proteins exhibited diminished clathrin uncoating activity in vitro. Together, these data provide genetic support for the long-suspected role of Hsc70 in clathrin-mediated endocytosis, at least in part by inhibiting the uncoating of CCVs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.