ArticleThe EMBO journal2002
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 68 citations in OpenAlex.
- Splicing the Difference: Harnessing the Complexity of the Transcriptome in Hematopoiesis.Experimental hematology · 2024Review
- Alternative splicing and related RNA binding proteins in human health and disease.Signal transduction and targeted therapy · 2024Review
- Blood Relatives: Splicing Mechanisms underlying Erythropoiesis in Health and Disease.F1000Research · 2018Review
- Alternative splicing as a regulator of development and tissue identity.Nature reviews. Molecular cell biology · 2017Review
- RNA splicing during terminal erythropoiesis.Current opinion in hematology · 2017Review
- Protein 4.1R Exon 16 3' Splice Site Activation Requires Coordination among TIA1, Pcbp1, and RBM39 during Terminal Erythropoiesis.Molecular and cellular biology · 2017Article
- Spliceosomal gene mutations in myelodysplasia: molecular links to clonal abnormalities of hematopoiesis.Genes & development · 2016Review
- The Role of Alternative Splicing in the Control of Immune Homeostasis and Cellular Differentiation.International journal of molecular sciences · 2015Review
- ZFP36L1 promotes monocyte/macrophage differentiation by repressing CDK6.Scientific reports · 2015Article
- HnRNP A1 tethers KSRP to an exon splicing silencer that inhibits an erythroid-specific splicing event in PU.1-induced erythroleukemia.American journal of cancer research · 2015Article
- Functional foods effective for hepatitis C: Identification of oligomeric proanthocyanidin and its action mechanism.World journal of hepatology · 2014Review
- A dynamic alternative splicing program regulates gene expression during terminal erythropoiesis.Nucleic acids research · 2014Article
- The RNA binding protein RBM38 (RNPC1) regulates splicing during late erythroid differentiation.PloS one · 2013Article
- Efficient in vivo manipulation of alternative pre-mRNA splicing events using antisense morpholinos in mice.The Journal of biological chemistry · 2011Article
- Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged.Genes & development · 2010Review
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- A computational approach for genome-wide mapping of splicing factor binding sites.Genome biology · 2009Article
- A proteomic comparison of immature and mature mouse gonadotrophs reveals novel differentially expressed nuclear proteins that regulate gonadotropin gene transcription and RNA splicing.Biology of reproduction · 2008Article
- RNA-binding proteins and post-transcriptional gene regulation.FEBS letters · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 6 institutions in 1 country.
Funding
Abstract
A physiologically important alternative pre-mRNA splicing switch, involving activation of protein 4.1R exon 16 (E16) splicing, is required for the establishment of proper mechanical integrity of the erythrocyte membrane during erythropoiesis. Here we identify a conserved exonic splicing silencer element (CE(16)) in E16 that interacts with hnRNP A/B proteins and plays a role in repression of E16 splicing during early erythropoiesis. Experiments with model pre-mRNAs showed that CE(16) can repress splicing of upstream introns, and that mutagenesis or replacement of CE(16) can relieve this inhibition. An affinity selection assay with biotinylated CE(16) RNA demonstrated specific binding of hnRNP A/B proteins. Depletion of hnRNP A/B proteins from nuclear extract significantly increased E16 inclusion, while repletion with recombinant hnRNP A/B restored E16 silencing. Most importantly, differentiating mouse erythroblasts exhibited a stage-specific activation of the E16 splicing switch in concert with a dramatic and specific down-regulation of hnRNP A/B protein expression. These findings demonstrate that natural developmental changes in hnRNP A/B proteins can effect physiologically important switches in pre-mRNA splicing.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.