ArticleThe EMBO journal2002
Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Structural analysis of a motor with increased mechanical output reveals new transitions in kinesin microtubule motility.Scientific reports · 2026Article
- Bound ion effects: Using machine learning method to study the kinesin Ncd's binding with microtubule.Biophysical journal · 2024Article
- Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes.Nature communications · 2024Article
- Maize VKS1 Regulates Mitosis and Cytokinesis During Early Endosperm Development.The Plant cell · 2019Article
- Kinesin-14 motor protein KIFC1 participates in DNA synthesis and chromatin maintenance.Cell death & disease · 2019Article
- The mitotic kinesin-14 KlpA contains a context-dependent directionality switch.Nature communications · 2017Article
- Working stroke of the kinesin-14, ncd, comprises two substeps of different direction.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Drosophila Ncd reveals an evolutionarily conserved powerstroke mechanism for homodimeric and heterodimeric kinesin-14s.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Remote control of myosin and kinesin motors using light-activated gearshifting.Nature nanotechnology · 2014Article
- High-resolution cryo-electron microscopy on macromolecular complexes and cell organelles.Protoplasma · 2014Review
- Common mechanistic themes for the powerstroke of kinesin-14 motors.Journal of structural biology · 2013Article
- Functional asymmetry in kinesin and dynein dimers.Biology of the cell · 2013Review
- Article
- Neck rotation and neck mimic docking in the noncatalytic Kar3-associated protein Vik1.The Journal of biological chemistry · 2012Article
- The ATPase pathway that drives the kinesin-14 Kar3Vik1 powerstroke.The Journal of biological chemistry · 2012Article
- Structural insights into WHAMM-mediated cytoskeletal coordination during membrane remodeling.The Journal of cell biology · 2012Article
- Biochemistry and cell biology of tau protein in neurofibrillary degeneration.Cold Spring Harbor perspectives in medicine · 2012Review
- Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.The Journal of cell biology · 2012Article
- Neck-motor interactions trigger rotation of the kinesin stalk.Scientific reports · 2012Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We used cryo-electron microscopy and image reconstruction to investigate the structure and microtubule-binding configurations of dimeric non-claret disjunctional (ncd) motor domains under various nucleotide conditions, and applied molecular docking using ncd's dimeric X-ray structure to generate a mechanistic model for force transduction. To visualize the alpha-helical coiled-coil neck better, we engineered an SH3 domain to the N-terminal end of our ncd construct (296-700). Ncd exhibits strikingly different nucleotide-dependent three-dimensional conformations and microtubule-binding patterns from those of conventional kinesin. In the absence of nucleotide, the neck adapts a configuration close to that found in the X-ray structure with stable interactions between the neck and motor core domain. Minus-end-directed movement is based mainly on two key events: (i) the stable neck-core interactions in ncd generate a binding geometry between motor and microtubule which places the motor ahead of its cargo in the minus-end direction; and (ii) after the uptake of ATP, the two heads rearrange their position relative to each other in a way that promotes a swing of the neck in the minus-end direction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.