ArticlePharmaceutical research2002
PepT1 mRNA expression is induced by starvation and its level correlates with absorptive transport of cefadroxil longitudinally in the rat intestine.
Article in Pharmaceutical research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 83 citations in OpenAlex.
- Development ofDiscover nano · 2026Article
- In Vitro In Vivo Extrapolation and Bioequivalence Prediction for Immediate-Release Capsules of Cefadroxil Based on a Physiologically-Based Pharmacokinetic ACAT Model.AAPS PharmSciTech · 2024Article
- Using Ex Vivo Porcine Jejunum to Identify Membrane Transporter Substrates: A Screening Tool for Early-Stage Drug Development.Biomedicines · 2020Article
- Physiological and therapeutic regulation of glucose homeostasis by upper small intestinal PepT1-mediated protein sensing.Nature communications · 2018Article
- Effect of coadministration of rifampicin on the pharmacokinetics of linezolid: clinical and animal studies.Journal of pharmaceutical health care and sciences · 2018Article
- Di- and tripeptide transport in vertebrates: the contribution of teleost fish models.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Review
- Species differences in the pharmacokinetics of cefadroxil as determined in wildtype and humanized PepT1 mice.Biochemical pharmacology · 2016Article
- Effect of dietary lead on intestinal nutrient transporters mRNA expression in broiler chickens.BioMed research international · 2015Article
- Cloning and molecular characterization of cationic amino acid transporter y⁺LAT1 in grass carp (Ctenopharyngodon idellus).Fish physiology and biochemistry · 2014Article
- Relevance of PepT1 in the intestinal permeability and oral absorption of cefadroxil.Pharmaceutical research · 2013Article
- The effect of fasting and refeeding on mRNA expression of PepT1 and gastrointestinal hormones regulating digestion and food intake in zebrafish (Danio rerio).Fish physiology and biochemistry · 2012Article
- Function and expression of the proton-coupled amino acid transporter PAT1 along the rat gastrointestinal tract: implications for intestinal absorption of gaboxadol.British journal of pharmacology · 2012Article
- Influence of fed-fasted state on intestinal PEPT1 expression and in vivo pharmacokinetics of glycylsarcosine in wild-type and Pept1 knockout mice.Pharmaceutical research · 2012Article
- Immunocytochemistry for bestatin and its application to drug accumulation studies in rat intestine and kidney.Journal of molecular histology · 2011Article
- Simulations of the nonlinear dose dependence for substrates of influx and efflux transporters in the human intestine.The AAPS journal · 2009Article
- Quantitative evaluation of PEPT1 contribution to oral absorption of cephalexin in rats.Pharmaceutical research · 2009Article
- Regulation of the oligopeptide transporter, PEPT-1, in DSS-induced rat colitis.Digestive diseases and sciences · 2007Article
- Prediction of glycylsarcosine transport in Caco-2 cell lines expressing PEPT1 at different levels.Pflugers Archiv : European journal of physiology · 2006Article
- Screening of the interaction between xenobiotic transporters and PDZ proteins.Pharmaceutical research · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo establish how closely intestinal transport activity for beta-lactam antibiotics is correlated with PepT1 expression, absolute expression level of PepT1 mRNA and transport activity were determined longitudinally in the small intestine of fed and starved rats.
methodsFor evaluation of absolute expression levels of PepTl mRNA, quantitative RT-PCR by LightCycler was used. The transport function was determined by quantifying the absorptive transport of cefadroxil across intestinal tissue sheets in a Ussing chamber.
resultsPepT1 mRNA expression was highest at the lower region and lowest at the upper region in the fed rats. The value of PepT1 was about 1/5-1/6 of that of GAPDH. The expression level in the starved rats was increased in all segments, but more profoundly in the upper region. Cefadroxil transport across intestinal tissue was higher in the lower region and lower in the upper region in fed rats, and increased in the upper region in starved rats. An excellent correlation was observed between expression levels and the permeability coefficients (r2 = 0.859, p < 0.05).
conclusionsThe intestinal transport of cefadroxil is directly proportional to PepT1 expression, suggesting that the PepT1 expression level in the rat small intestine is the major determinant of the absorption of peptide-like compounds.
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