Evidence map›Paper›PMID 12423064›Full record

ArticleAAPS pharmSci2002

Cloning and characterization of the rat multidrug resistance-associated protein 1.

Ziping Yang, Cheryl S W Li, Danny D Shen, Rodney J Y Ho

Open access · bronzeAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in AAPS pharmSci, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Ziping YangDepartment of Pharmaceutics, University of Washington, Seattle, WA, USA.
Cheryl S W Li
Danny D Shen
Rodney J Y Ho
University of Washington · US

Funding

CENTRAL NERVOUS SYSTEM STRATEGIES TARGETING THE HIV SANCR01NS039178 · NINDS · UNIVERSITY OF UTAH · PI ANDERSON, BRADLEY D · 1999 to 2003
$1.6M
Polymorphism of Multi-Drug-Resistance Protein-1R01GM062883 · NIGMS · UNIVERSITY OF WASHINGTON · PI HO, RODNEY J.Y. · 2002 to 2005
$1.3M
KINETICS &MECHANISM OF MOTHER-TO-FETUS HIV TRANSMISSIONR01HL056548 · NHLBI · UNIVERSITY OF WASHINGTON · PI HO, RODNEY J.Y. · 1996 to 2000
$474k
CNS DELIVERY OF ALKANOATE ANTIEPILEPTICSR01NS030738 · NINDS · UNIVERSITY OF WASHINGTON · PI SHEN, DANNY D · 1992 to 1999
–
NHLBI NIH HHS HL56548NIAID NIH HHS AI35814NIGMS NIH HHS GM62883NINDS NIH HHS NS30738NINDS NIH HHS NS39178
6 · The paper itself

Abstract

Multidrug resistance-associated protein 1 (MRP1) was originally shown to confer resistance of human tumor cells to a broad range of natural product anticancer drugs. MRP1 has also been shown to mediate efflux transport of glutathione and glucuronide conjugates of drugs and endogenous substrates. An ortholog of MRP1 in the mouse has been cloned and characterized. Significant functional differences between murine and human MRP1 have been noted. Since drug disposition and pharmacology studies often are conducted in rats, there is a need to clone and characterize the rat ortholog of MRP1. We isolated a rat MRP1 (rMRP1) cDNA from rat brain astrocytes, characterized its coding sequences, and verified the transport activity of the protein expressed in MRP1 cDNA-transfected Madin-Darby canine kidney (MDCK) cells. Our results showed that rMRP1 has a coding sequence of 4599 bp, which predicts a polypeptide of 1533 amino acids with an apparent molecular weight of 190 kd by Western immunoblot analysis. rMRP1-transfected MDCK cells are capable of efflux transport of a fluorescent MRP1 marker - calcein - that is inhibitable by known MRP1 inhibitors, indomethacin, and MK571. Sequence analysis indicates that rMRP1 is more closely related to mouse MRP1 than human MRP1.

Indexed as

Amino Acid SequenceAnimalsAstrocytesATP-Binding Cassette, Sub-Family C ProteinsBase SequenceBrainCell LineCells, CulturedCloning, MolecularDNA, ComplementaryDogsFemaleHumansKidneyMiceMolecular Sequence DataATP-Binding Cassette, Sub-Family C ProteinsDNA, Complementarymultidrug resistance-associated protein 1

Identifiers

PMID12423064
PMCPMC2751354
OpenAlexW2086262105

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.