ArticleGenome research2002
Genomic signature tags (GSTs): a system for profiling genomic DNA.
Article in Genome research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Genetic dissection of platelet function in health and disease using systems biology.Hematology/oncology clinics of North America · 2013Review
- Article
- Next-generation DNA sequencing of paired-end tags (PET) for transcriptome and genome analyses.Genome research · 2009Review
- Functional analysis of MmeI from methanol utilizer Methylophilus methylotrophus, a subtype IIC restriction-modification enzyme related to type I enzymes.Applied and environmental microbiology · 2009Article
- Classification and regression tree (CART) analyses of genomic signatures reveal sets of tetramers that discriminate temperature optima of archaea and bacteria.Archaea (Vancouver, B.C.) · 2008Article
- Metagenome fragment classification using N-mer frequency profiles.Advances in bioinformatics · 2008Article
- Binding of MmeI restriction-modification enzyme to its specific recognition sequence is stimulated by S-adenosyl-L-methionine.Molecular biotechnology · 2007Article
- Review
- Use of single-point genome signature tags as a universal tagging method for microbial genome surveys.Applied and environmental microbiology · 2006Article
- A high throughput method for genome-wide analysis of retroviral integration.Nucleic acids research · 2006Article
- Discovery of functional noncoding elements by digital analysis of chromatin structure.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Genomic representations using concatenates of Type IIB restriction endonuclease digestion fragments.Nucleic acids research · 2004Article
- A genomic selection strategy to identify accessible and dimerization blocking targets in the 5'-UTR of HIV-1 RNA.Nucleic acids research · 2004Article
- Application of DNA microarrays to study the evolutionary genomics of Yersinia pestis and Yersinia pseudotuberculosis.Genome research · 2003Article
- Current awareness on comparative and functional genomics.Comparative and functional genomics · 2003Article
- Digital karyotyping.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genomic signature tags (GSTs) are the products of a method we have developed for identifying and quantitatively analyzing genomic DNAs. The DNA is initially fragmented with a type II restriction enzyme. An oligonucleotide adaptor containing a recognition site for MmeI, a type IIS restriction enzyme, is then used to release 21-bp tags from fixed positions in the DNA relative to the sites recognized by the fragmenting enzyme. These tags are PCR-amplified, purified, concatenated, and then cloned and sequenced. The tag sequences and abundances are used to create a high-resolution GST sequence profile of the genomic DNA. GSTs are shown to be long enough for use as oligonucleotide primers to amplify adjacent segments of the DNA, which can then be sequenced to provide additional nucleotide information or used as probes to identify specific clones in metagenomic libraries. GST analysis of the 4.7-Mb Yersinia pestis EV766 genome using BamHI as the fragmenting enzyme and NlaIII as the tagging enzyme validated the precision of our approach. The GST profile predicts that this strain has several changes relative to the archetype CO92 strain, including deletion of a 57-kb region of the chromosome known to be an unstable pathogenicity island.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.