ArticleBMC neuroscience2002
Evidence for non-independent gating of P2X2 receptors expressed in Xenopus oocytes.
Article in BMC neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- Generalised Hodgkin-Huxley model captures human P2X and AMPA receptor currents.The Journal of physiology · 2026Article
- Copper Increases the Cooperative Gating of Rat P2X2a Receptor Channels.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Article
- Structural titration of receptor ion channel GLIC gating by HS-AFM.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Direct visualization of ion-channel gating in a native environment.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- P2X2 Dominant Deafness Mutations Have No Negative Effect on Wild-Type Isoform: Implications for Functional Rescue and in Deafness Mechanism.Frontiers in molecular neuroscience · 2017Article
- Human slack potassium channel mutations increase positive cooperativity between individual channels.Cell reports · 2014Article
- Heteromeric assembly of P2X subunits.Frontiers in cellular neuroscience · 2013Review
- Expression-dependent pharmacology of transient receptor potential vanilloid subtype 1 channels in Xenopus laevis oocytes.Channels (Austin, Tex.) · 2013Article
- Unraveling subunit cooperativity in homotetrameric HCN2 channels.Biophysical journal · 2012Article
- Coupled gating of skeletal muscle ryanodine receptors is modulated by Ca2+, Mg2+, and ATP.American journal of physiology. Cell physiology · 2012Article
- Molecular and functional properties of P2X receptors--recent progress and persisting challenges.Purinergic signalling · 2012Review
- P2X4 receptors interact with both P2X2 and P2X7 receptors in the form of homotrimers.British journal of pharmacology · 2011Article
- Voltage-dependent facilitation of Cx46 hemichannels.American journal of physiology. Cell physiology · 2010Article
- Analysis of assembly and trafficking of native P2X4 and P2X7 receptor complexes in rodent immune cells.The Journal of biological chemistry · 2009Article
- In situ recording from gut pacemaker cells.Pflugers Archiv : European journal of physiology · 2008Article
- Trace metals in the brain: allosteric modulators of ligand-gated receptor channels, the case of ATP-gated P2X receptors.European biophysics journal : EBJ · 2008Review
- P2X1 and P2X4 receptor currents in mouse macrophages.British journal of pharmacology · 2007Article
- Activation kinetics of single P2X receptors.Purinergic signalling · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundP2X2 receptor is an ATP-activated ion channel which is widely expressed in the nervous system, and mediates synaptic transmission.
resultsWe recorded currents of P2X2 receptors expressed in Xenopus oocytes from outside-out patches and have found that currents recorded from patches containing a single or multiple P2X2 channels differ in a manner suggesting positive cooperativity. First, the currents from multichannel patches exhibit simultaneous transitions more frequently than predicted from the activity of independent channels. Second, the mean open lifetime at the current level of a single channel in a multichannel burst is about six times longer than the open time of currents from single channel patches, a trend opposite to what is expected of independent channels. These results indicate that the channels have positive cooperativity and that the longer opening is due to a slower closing rate. Third, from kinetic analysis the likelihood of the cooperative model is significantly larger than that of the independent model. Fourth, the open channel noise of currents from patches containing multiple channels is less than half that from a single channel, which is consistent with the channel properties being different when they are active in groups.
conclusionTaken together, our results suggest that P2X2 receptors are non-independent, but interact with positive cooperativity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.