ArticleMolecular and cellular biology2002
Phosphorylation of Tyr342 in the linker region of Syk is critical for Fc epsilon RI signaling in mast cells.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Phosphorylation at Y317 on Syk negatively regulates both ITAM- and hemITAM-mediated signaling and function in platelets.Blood advances · 2025Article
- Exploring the anti-anaphylaxis potential of natural products: A Review.Inflammopharmacology · 2025Review
- TULA Proteins in Men, Mice, Hens, and Lice: Welcome to the Family.International journal of molecular sciences · 2023Review
- Structures and Anti-Allergic Activities of Natural Products from Marine Organisms.Marine drugs · 2023Review
- TULA-Family Regulators of Platelet Activation.International journal of molecular sciences · 2022Review
- Interaction of DJ-1 with Lyn is essential for IgE-mediated stimulation of human mast cells.The Journal of allergy and clinical immunology · 2018Article
- TULA-2 Protein Phosphatase Suppresses Activation of Syk through the GPVI Platelet Receptor for Collagen by Dephosphorylating Tyr(P)346, a Regulatory Site of Syk.The Journal of biological chemistry · 2016Article
- DJ-1 regulates mast cell activation and IgE-mediated allergic responses.The Journal of allergy and clinical immunology · 2013Article
- A mechanistic model of early FcεRI signaling: lipid rafts and the question of protection from dephosphorylation.PloS one · 2012Article
- Shp2 activates Fyn and Ras to regulate RBL-2H3 mast cell activation following FcεRI aggregation.PloS one · 2012Article
- Regulation and function of syk tyrosine kinase in mast cell signaling and beyond.Journal of signal transduction · 2011Article
- Mast cell signaling: the role of protein tyrosine kinase Syk, its activation and screening methods for new pathway participants.FEBS letters · 2010Review
- Tyrosines in the carboxyl terminus regulate Syk kinase activity and function.The Journal of biological chemistry · 2010Article
- Small interfering RNA screen for phosphatases involved in IgE-mediated mast cell degranulation.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- The SYK tyrosine kinase: a crucial player in diverse biological functions.Nature reviews. Immunology · 2010Review
- FcepsilonRI-induced activation by low antigen concentrations results in nuclear signals in the absence of degranulation.Molecular immunology · 2009Article
- Syk tyrosine 317 negatively regulates osteoclast function via the ubiquitin-protein isopeptide ligase activity of Cbl.The Journal of biological chemistry · 2009Article
- Syk and pTyr'd: Signaling through the B cell antigen receptor.Biochimica et biophysica acta · 2009Review
- The tyrosine kinase network regulating mast cell activation.Immunological reviews · 2009Review
- The kinase Syk as an adaptor controlling sustained calcium signalling and B-cell development.The EMBO journal · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The linker region of Syk and ZAP70 tyrosine kinases plays an important role in regulating their function. There are three conserved tyrosines in this linker region; Tyr317 of Syk and its equivalent residue in ZAP70 were previously shown to negatively regulate the function of Syk and ZAP70. Here we studied the roles of the other two tyrosines, Tyr342 and Tyr346 of Syk, in Fc epsilon RI-mediated signaling. Antigen stimulation resulted in Tyr342 phosphorylation in mast cells. Syk with Y342F mutation failed to reconstitute Fc epsilon RI-initiated histamine release. In the Syk Y342F-expressing cells there was dramatically impaired receptor-induced phosphorylation of multiple signaling molecules, including LAT, SLP-76, phospholipase C-gamma2, but not Vav. Compared to wild-type Syk, Y342F Syk had decreased binding to phosphorylated immunoreceptor tyrosine-based activation motifs and reduced kinase activity. Surprisingly, mutation of Tyr346 had much less effect on Fc epsilon RI-dependent mast cell degranulation. An anti-Syk-phospho-346 tyrosine antibody indicated that antigen stimulation induced only a very minor increase in the phosphorylation of this tyrosine. Therefore, Tyr342, but not Tyr346, is critical for regulating Syk in mast cells and the function of these tyrosines in immune receptor signaling appears to be different from what has been previously reported for the equivalent residues of ZAP70.
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