ArticleThe Plant cell2002
The Chlamydomonas reinhardtii organellar genomes respond transcriptionally and post-transcriptionally to abiotic stimuli.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 58 citations in OpenAlex.
- Chloroplast Genetic Engineering of a Unicellular Green Alga Haematococcus pluvialis with Expression of an Antimicrobial Peptide.Marine biotechnology (New York, N.Y.) · 2020Article
- MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii.Scientific reports · 2016Article
- Multiple regulatory mechanisms in the chloroplast of green algae: relation to hydrogen production.Photosynthesis research · 2015Article
- Fermentation metabolism and its evolution in algae.Frontiers in plant science · 2013Article
- System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium.Molecular & cellular proteomics : MCP · 2012Article
- Transcriptomic response of the red tide dinoflagellate, Karenia brevis, to nitrogen and phosphorus depletion and addition.BMC genomics · 2011Article
- Organization and expression of organellar genomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- High quality metabolomic data for Chlamydomonas reinhardtii.Plant methods · 2008Article
- Chlamydomonas reinhardtii, a model system for functional validation of abiotic stress responsive genes.Planta · 2007Article
- Article
- Evidence for regulatory function of nucleus-encoded factors on mRNA stabilization and translation in the chloroplast.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Distinct roles for the 5' and 3' untranslated regions in the degradation and accumulation of chloroplast tufA mRNA: identification of an early intermediate in the in vivo degradation pathway.Plant molecular biology · 2007Article
- The complete chloroplast genome sequence of the chlorophycean green alga Scenedesmus obliquus reveals a compact gene organization and a biased distribution of genes on the two DNA strands.BMC evolutionary biology · 2006Article
- Sulfur and primary production in aquatic environments: an ecological perspective.Photosynthesis research · 2005Review
- Microarray profiling of plastid gene expression in a unicellular red alga, Cyanidioschyzon merolae.Plant molecular biology · 2005Article
- Microarray analysis confirms the specificity of a Chlamydomonas reinhardtii chloroplast RNA stability mutant.Plant physiology · 2005Article
- Paths toward algal genomics.Plant physiology · 2005Review
- Chloroplast RNA processing and stability.Photosynthesis research · 2004Article
- The translational apparatus of Chlamydomonas reinhardtii chloroplast.Photosynthesis research · 2004Article
- Chlamydomonas reinhardtii at the crossroads of genomics.Eukaryotic cell · 2003Review
Corrections and comments
- Retraction · 2004-03-01Falsification/Fabrication of Data · Investigation by Company/Institution · Manipulation of Results · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author · Results Not Reproducible · · Jason Lilly has institutional finding of misconduct;
- Commented on by
- RetractedRetraction.2004
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Chlamydomonas reinhardtii plastid and mitochondrial transcriptomes were surveyed for changes in RNA profiles resulting from growth in 12 culture conditions representing 8 abiotic stimuli. Organellar RNA abundance exhibited marked changes during nutrient stress and exposure to UV light, as revealed by both RNA gel blot and DNA microarray analyses. Of particular note were large increases in tufA and clpP transcript abundance during nutrient limitation. Phosphate and sulfur limitation resulted in the most global, yet opposite, effects on organellar RNA abundance, changes that were dissected further using run-on transcription assays. Removal of sulfate from the culture medium, which is known to reduce photosynthesis, resulted in 2-fold to 10-fold decreases in transcription rates, which were reflected in lower RNA abundance. The decrease in transcriptional activity was completely reversible and recovered to twice the control level after sulfate replenishment. Conversely, phosphate limitation resulted in a twofold to threefold increase in RNA abundance that was found to be a post-transcriptional effect, because it could be accounted for by increased RNA stability. This finding is consistent with the known metabolic slowdown under phosphate stress. Additionally, inhibitor studies suggested that unlike those in higher plants, Chlamydomonas chloroplasts lack a nucleus-encoded plastid RNA polymerase. The apparently single type of polymerase could contribute to the rapid and genome-wide transcriptional responses observed within the chloroplast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.