ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2002
Differential regulation of active zone density during long-term strengthening of Drosophila neuromuscular junctions.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 94 citations in OpenAlex.
- Distinct input-specific mechanisms enable presynaptic homeostatic plasticity.Science advances · 2025Article
- What do the mushroom bodies do for the insect brain? Twenty-five years of progress.Learning & memory (Cold Spring Harbor, N.Y.) · 2024Article
- The Architecture of the Presynaptic Release Site.Advances in neurobiology · 2023Article
- Structural Remodeling of Active Zones Is Associated with Synaptic Homeostasis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Presynaptic active zones of mammalian neuromuscular junctions: Nanoarchitecture and selective impairments in aging.Neuroscience research · 2018Review
- Serotonergic Modulation Enables Pathway-Specific Plasticity in a Developing Sensory Circuit in Drosophila.Neuron · 2017Article
- Kek-6: A truncated-Trk-like receptor for Drosophila neurotrophin 2 regulates structural synaptic plasticity.PLoS genetics · 2017Article
- Fife organizes synaptic vesicles and calcium channels for high-probability neurotransmitter release.The Journal of cell biology · 2017Article
- Illuminating Brain Activities with Fluorescent Protein-Based Biosensors.Chemosensors (Basel, Switzerland) · 2017Article
- A Novel, Noncanonical BMP Pathway Modulates Synapse Maturation at the Drosophila Neuromuscular Junction.PLoS genetics · 2016Article
- Synergistic interactions between Drosophila orthologues of genes spanned by de novo human CNVs support multiple-hit models of autism.PLoS genetics · 2015Article
- Postsynaptic actin regulates active zone spacing and glutamate receptor apposition at the Drosophila neuromuscular junction.Molecular and cellular neurosciences · 2014Article
- Postsynaptic glutamate receptors regulate local BMP signaling at the Drosophila neuromuscular junction.Development (Cambridge, England) · 2014Article
- The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles.The Journal of cell biology · 2013Article
- Mushroom body miscellanea: transgenic Drosophila strains expressing anatomical and physiological sensor proteins in Kenyon cells.Frontiers in neural circuits · 2013Article
- Neuronal influence on peripheral circadian oscillators in pupal Drosophila prothoracic glands.Nature communications · 2012Article
- Active zone density is conserved during synaptic growth but impaired in aged mice.The Journal of comparative neurology · 2012Article
- Presynaptic Active Zone Density during Development and Synaptic Plasticity.Frontiers in molecular neuroscience · 2012Article
- Neurexins and neuroligins: recent insights from invertebrates.Molecular neurobiology · 2011Review
- Fighting the famine with an amine: synaptic strategies for smart search.Nature neuroscience · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we established a transgenic Ca2+ imaging technique in Drosophila that enabled us to target the Ca2+ sensor protein yellow Cameleon-2 specifically to larval neurons. This noninvasive method allowed us to measure evoked Ca2+ signals in presynaptic terminals of larval neuromuscular junctions (NMJs). We combined transgenic Ca2+ imaging with electrophysiological recordings and morphological examinations of larval NMJs to analyze the mechanisms underlying persistently enhanced evoked vesicle release in two independent mutants. We show that persistent strengthening of junctional vesicle release relies on the recruitment of additional active zones, the spacing of which correlated with the evoked presynaptic Ca2+ dynamics of individual presynaptic terminals. Knock-out mutants of the postsynaptic glutamate receptor (GluR) subunit DGluR-IIA, which showed a reduced quantal size, developed NMJs with a smaller number of presynaptic boutons but a strong compensatory increase in the density of active zones. This resulted in an increased evoked vesicle release on single action potentials and larger evoked Ca2+ signals within individual boutons; however, the transmission of higher frequency stimuli was strongly depressed. A second mutant (pabp(P970)/+), which showed enhanced evoked vesicle release triggered by elevated subsynaptic protein synthesis, developed NMJs with an increased number of presynaptic boutons and active zones; however, the density of active zones was maintained at a value typical for wild-type animals. This resulted in wild-type evoked Ca2+ signals but persistently strengthened junctional signal transmission. These data suggest that the consolidation of strengthened signal transmission relies not only on the recruitment of active zones but also on their equal distribution in newly grown boutons.
Indexed as
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12417665PMC6758049W1808236879What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.