ArticleThe Journal of experimental medicine2002
Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway.
Article in The Journal of experimental medicine, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Spatiotemporal expression and coexpression patterns of SRPK1 in the human brain: A neurodevelopmental perspective.Brain and behavior · 2024Article
- Article
- Effects of ginsenoside Rg3 on apoptosis in A375.S2 melanoma cells.Translational cancer research · 2019Article
- Autoantigens as Partners in Initiation and Propagation of Autoimmune Rheumatic Diseases.Annual review of immunology · 2016Review
- Synthesis and Biological Evaluation of Novel Dehydroabietic Acid Derivatives Conjugated with Acyl-Thiourea Peptide Moiety as Antitumor Agents.International journal of molecular sciences · 2015Article
- A Multi-Breed Genome-Wide Association Analysis for Canine Hypothyroidism Identifies a Shared Major Risk Locus on CFA12.PloS one · 2015Article
- Specific post-translational histone modifications of neutrophil extracellular traps as immunogens and potential targets of lupus autoantibodies.Arthritis research & therapy · 2012Article
- Acetylation and phosphorylation of SRSF2 control cell fate decision in response to cisplatin.The EMBO journal · 2011Article
- The N-terminal fragment from caspase-cleaved serine/arginine protein-specific kinase2 (SRPK2) translocates into the nucleus and promotes apoptosis.The Journal of biological chemistry · 2011Article
- CD4+ T cells sensitized by vascular smooth muscle induce vasculitis, and interferon gamma is critical for the initiation of vascular pathology.The American journal of pathology · 2010Article
- Inhibitors of MAPK pathway ERK1/2 or p38 prevent the IL-1{beta}-induced up-regulation of SRP72 autoantigen in Jurkat cells.The Journal of biological chemistry · 2010Article
- BITC Sensitizes Pancreatic Adenocarcinomas to TRAIL-induced Apoptosis.Cancer growth and metastasis · 2010Article
- Interaction of Akt-phosphorylated SRPK2 with 14-3-3 mediates cell cycle and cell death in neurons.The Journal of biological chemistry · 2009Article
- Serine/arginine protein-specific kinase 2 promotes leukemia cell proliferation by phosphorylating acinus and regulating cyclin A1.Cancer research · 2008Article
- Dsk1p kinase phosphorylates SR proteins and regulates their cellular localization in fission yeast.The Biochemical journal · 2007Article
- Cell stress modulates the function of splicing regulatory protein RBM4 in translation control.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Multiplexed protein measurement: technologies and applications of protein and antibody arrays.Nature reviews. Drug discovery · 2006Review
- Proteolytic cleavage of the catalytic subunit of DNA-dependent protein kinase during poliovirus infection.Journal of virology · 2004Article
- Many cuts to ruin: a comprehensive update of caspase substrates.Cell death and differentiation · 2003Review
- Resistance to platinum-containing chemotherapy in testicular germ cell tumors is associated with downregulation of the protein kinase SRPK1.Neoplasia (New York, N.Y.)Article
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Authors and funding
6 authors.
Funding
Abstract
Using human autoimmune sera as molecular probes, we previously described the association of phosphorylated serine/arginine splicing factors (SR splicing factors) with the U1-small nuclear ribonucleoprotein (U1-snRNP) and U3-small nucleolar RNP (snoRNP) in apoptotic cells. SR proteins are highly conserved autoantigens whose activity is tightly regulated by reversible phosphorylation of serine residues by at least eight different SR protein kinase kinases (SRPKs), including SRPK1, SRPK2, and the scleroderma autoantigen topoisomerase I. In this report, we demonstrate that only one of the known SRPKs, SRPK1, is associated with the U1-snRNP autoantigen complex in healthy and apoptotic cells. SRPK1 is activated early during apoptosis, followed by caspase-mediated proteolytic inactivation at later time points. SRPKs are cleaved in vivo after multiple apoptotic stimuli, and cleavage can be inhibited by overexpression of bcl-2 and bcl-x(L), and by exposure to soluble peptide caspase inhibitors. Incubation of recombinant caspases with in vitro-translated SRPKs demonstrates that SRPK1 and SRPK2 are in vitro substrates for caspases-8 and -9, respectively. In contrast, topoisomerase I is cleaved by downstream caspases (-3 and -6). Since each of these SRPKs sits at a distinct checkpoint in the caspase cascade, SRPKs may serve an important role in signaling pathways governing apoptosis, alternative mRNA splicing, SR protein trafficking, RNA stability, and possibly the generation of autoantibodies directed against splicing factors.
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