ArticleNature biotechnology2002
Boosting heterologous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences.
Article in Nature biotechnology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 166 citations in OpenAlex.
- Novel synthetic inducible promoters controlling gene expression during water-deficit stress with green tissue specificity in transgenic poplar.Plant biotechnology journal · 2024Article
- Fusing the 3'UTR of seed storage protein genes leads to massive recombinant protein accumulation in seeds.Scientific reports · 2023Article
- Seed- and leaf-based expression of FGF21-transferrin fusion proteins for oral delivery and treatment of non-alcoholic steatohepatitis.Frontiers in plant science · 2022Article
- Plant Platforms for Efficient Heterologous Protein Production.Biotechnology and bioprocess engineering : BBE · 2021Review
- Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update.Molecular biotechnology · 2020Review
- Transformation strategies for stable expression of complex hetero-multimeric proteins like secretory immunoglobulin A in plants.Plant biotechnology journal · 2019Article
- A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants.Frontiers in plant science · 2019Review
- Russell-Like Bodies in Plant Seeds Share Common Features With Prolamin Bodies and Occur Upon Recombinant Protein Production.Frontiers in plant science · 2019Article
- Transgenic Nicotiana tabacum seeds expressing the Mycobacterium tuberculosis Alanine- and Proline-rich antigen.AMB Express · 2018Article
- In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter.PloS one · 2018Article
- High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza.Transgenic research · 2017Article
- Nattokinase: An Oral Antithrombotic Agent for the Prevention of Cardiovascular Disease.International journal of molecular sciences · 2017Review
- The pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in Arabidopsis thaliana plants.World journal of microbiology & biotechnology · 2016Article
- Recombinant IgA production for mucosal passive immunization, advancing beyond the hurdles.Cellular and molecular life sciences : CMLS · 2016Review
- Plants as Factories for Human Pharmaceuticals: Applications and Challenges.International journal of molecular sciences · 2015Review
- Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds.Nature biotechnology · 2015Article
- Tobacco seeds as efficient production platform for a biologically active anti-HBsAg monoclonal antibody.Transgenic research · 2015Article
- Ectopic expression of human acidic fibroblast growth factor 1 in the medicinal plant, Salvia miltiorrhiza, accelerates the healing of burn wounds.BMC biotechnology · 2014Article
- Single-domain antibodies targeting neuraminidase protect against an H5N1 influenza virus challenge.Journal of virology · 2014Article
- The dynamic behavior of storage organelles in developing cereal seeds and its impact on the production of recombinant proteins.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the past decade, several high value proteins have been produced in different transgenic plant tissues such as leaves, tubers, and seeds. Despite recent advances, many heterologous proteins accumulate to low concentrations, and the optimization of expression cassettes to make in planta production and purification economically feasible remains critical. Here, the regulatory sequences of the seed storage protein gene arcelin 5-I (arc5-I) of common bean (Phaseolus vulgaris) were evaluated for producing heterologous proteins in dicotyledonous seeds. The murine single chain variable fragment (scFv) G4 (ref. 4) was chosen as model protein because of the current industrial interest in producing antibodies and derived fragments in crops. In transgenic Arabidopsis thaliana seed stocks, the scFv under control of the 35S promoter of the cauliflower mosaic virus (CaMV) accumulated to approximately 1% of total soluble protein (TSP). However, a set of seed storage promoter constructs boosted the scFv accumulation to exceptionally high concentrations, reaching no less than 36.5% of TSP in homozygous seeds. Even at these high concentrations, the scFv proteins had antigen-binding activity and affinity similar to those produced in Escherichia coli. The feasibility of heterologous protein production under control of arc5-I regulatory sequences was also demonstrated in Phaseolus acutifolius, a promising crop for large scale production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.