ArticleProceedings of the National Academy of Sciences of the United States of America2002
Identification of E2A target genes in B lymphocyte development by using a gene tagging-based chromatin immunoprecipitation system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 81 citations in OpenAlex.
- The advances of E2A-PBX1 fusion in B-cell acute lymphoblastic Leukaemia.Annals of hematology · 2024Review
- Relationship between the structure and function of the transcriptional regulator E2A.Journal of biological research (Thessalonike, Greece) · 2021Review
- Structural insights into TAZ2 domain-mediated CBP/p300 recruitment by transactivation domain 1 of the lymphopoietic transcription factor E2A.The Journal of biological chemistry · 2020Article
- YY1 binds to the E3' enhancer and inhibits the expression of the immunoglobulin κ gene via epigenetic modifications.Immunology · 2018Article
- Paradoxical role of Id proteins in regulating tumorigenic potential of lymphoid cells.Frontiers of medicine · 2018Review
- [Merkel cell carcinoma: cutaneous manifestation of a highly malignant pre-/pro-B cell neoplasia? : Novel concept about the cellular origin of Merkel cell carcinoma].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2017Review
- Functional redundancy between the transcriptional activation domains of E2A is mediated by binding to the KIX domain of CBP/p300.Nucleic acids research · 2014Article
- Pre-B cell receptor signaling induces immunoglobulin κ locus accessibility by functional redistribution of enhancer-mediated chromatin interactions.PLoS biology · 2014Article
- Biochemical and phosphoproteomic analysis of the helix-loop-helix protein E47.Molecular and cellular biology · 2012Article
- Article
- EBF proteins participate in transcriptional regulation of Xenopus muscle development.Developmental biology · 2011Article
- Forced expression of cyclin-dependent kinase 6 confers resistance of pro-B acute lymphocytic leukemia to Gleevec treatment.Molecular and cellular biology · 2011Article
- E2A is a transcriptional regulator of CD38 expression in chronic lymphocytic leukemia.Leukemia · 2011Article
- Id1 has a physiological role in regulating early B lymphopoiesis.Cellular & molecular immunology · 2011Article
- E proteins and the regulation of early lymphocyte development.Immunological reviews · 2010Review
- Gatekeepers of intestinal inflammation.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2010Review
- Distinct roles for E12 and E47 in B cell specification and the sequential rearrangement of immunoglobulin light chain loci.The Journal of experimental medicine · 2009Article
- Article
- Ebf1-mediated down-regulation of Id2 and Id3 is essential for specification of the B cell lineage.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Regulation of lymphocyte development by cell-type-specific interpretation of Notch signals.Molecular and cellular biology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The transcription factors encoded by the E2A gene are known to be essential for B lymphocyte development, and ectopic expression or gene inactivation studies have revealed several potential lineage-specific E2A target genes. However, it remains unknown whether these target genes are directly regulated by E2A at the transcriptional level. We therefore generated mice carrying an affinity-tagged E2A knock-in allele to provide a system for the direct elucidation of E2A target genes based on E2A binding to target regulatory regions. Abelson-transformed pre-B cell lines derived from these mice were used in chromatin immunoprecipitation experiments to identify regulatory sequences bound by E2A in the context of an early B lymphocyte environment. Significant E2A binding was detected at the promoters and enhancers of several essential B-lineage genes, including the Igkappa intronic and 3' enhancers, lambda5 and VpreB surrogate light chain promoters, the EBF locus promoter region, and the mb-1 (Igalpha) promoter. Low levels of E2A binding were observed at several other lymphoid-restricted regulatory regions including the Ig heavy chain (IgH) intronic enhancer, the IgH 3' enhancers hs3b/hs4, the RAG-2 enhancer, and the 5' regions of the B29 and TdT loci. An E2A target gene, the predicted butyrophilin-like gene NG9 (BTL-II), was also identified by using a chromatin immunoprecipitation-based cloning strategy. In summary, our studies have provided evidence that E2A is directly involved in the transcriptional regulation of a number of early B-lineage genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.