ArticleClinical and diagnostic laboratory immunology2002
Leishmania major-like antigen for specific and sensitive serodiagnosis of human and canine visceral leishmaniasis.
Article in Clinical and diagnostic laboratory immunology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 61 citations in OpenAlex.
- Global Landscape of Molecular and Immunological Diagnostic Tests for Human Leishmaniasis: A Systematic Review and Meta-Analysis.Pathogens (Basel, Switzerland) · 2025Pooled it
- Systematic review on antigens for serodiagnosis of visceral leishmaniasis, with a focus on East Africa.PLoS neglected tropical diseases · 2019Pooled it
- Recombinant LPG3 Protein From Leishmania chagasi as an Antigen for the Diagnosis of Canine Visceral Leishmaniasis.Tropical medicine & international health : TM & IH · 2026Article
- Sire risk factors for vertical transmission of Leishmania infantum by the dam.PLoS neglected tropical diseases · 2026Article
- Clinical anemia predicts dermal parasitism and reservoir infectiousness during progressive visceral leishmaniosis.PLoS neglected tropical diseases · 2024Article
- Cytokine and phenotypic cell profiles in human cutaneous leishmaniasis caused by Leishmania donovani.PloS one · 2023Article
- Quantification of Soluble or Insoluble Fractions ofInternational journal of analytical chemistry · 2020Article
- Prevalidation of an ELISA for Detection of a New Clinical Entity:International journal of analytical chemistry · 2020Article
- Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology.Parasites & vectors · 2017Article
- Comparative immune study on cutaneous leishmaniasis patients with single and multiple sores.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2015Article
- Evaluation of conjunctival swab as a mass-screening tool for molecular diagnosis of canine visceral leishmaniasis.Parasitology research · 2015Article
- Novel recombinant multiepitope proteins for the diagnosis of asymptomatic leishmania infantum-infected dogs.PLoS neglected tropical diseases · 2015Article
- Epitope mapping of the HSP83.1 protein of Leishmania braziliensis discloses novel targets for immunodiagnosis of tegumentary and visceral clinical forms of leishmaniasis.Clinical and vaccine immunology : CVI · 2014Article
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- Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil.PloS one · 2013Article
- Canine skin and conjunctival swab samples for the detection and quantification of Leishmania infantum DNA in an endemic urban area in Brazil.PLoS neglected tropical diseases · 2012Article
- Identification of proteins in promastigote and amastigote-like Leishmania using an immunoproteomic approach.PLoS neglected tropical diseases · 2012Article
- In situ hybridisation for the detection of Leishmania species in paraffin wax-embedded canine tissues using a digoxigenin-labelled oligonucleotide probe.The Veterinary record · 2011Article
- Specific serodiagnosis of canine visceral leishmaniasis using Leishmania species ribosomal protein extracts.Clinical and vaccine immunology : CVI · 2009Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An antigen (LMS) prepared from Leishmania major-like promastigotes was used in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of human and dog visceral leishmaniasis. The results were compared with those from the indirect immunofluorescent antibody test (IFAT). A total of 1822 canine sera were tested, including sera from dogs with visceral leishmaniasis, transmissible venereal tumors, ehrlichiosis, rickettsiosis, or Chagas' disease and sera from healthy dogs. The antigen was also tested with 227 samples of human sera, including sera from patients with visceral, cutaneous, or diffuse cutaneous leishmaniasis and from noninfected individuals, as well as sera from patients with Chagas' disease, toxoplasmosis, rickettsiosis, hepatitis B, schistosomiasis, ascaridiasis, malaria, rheumatoid factor, leprosy and rheumatoid factor, tuberculosis, or leprosy. All dogs and all human patients had a clinical and/or serological and/or parasitological diagnosis. For detecting antibodies in sera from dogs with leishmaniasis, the antigen showed a sensitivity of 98%, specificity of 95%, and concordance of 93% and when used for detecting antibodies in human sera presented a sensitivity of 92%, specificity of 100%, and concordance of 92%. Comparison between ELISA and IFAT demonstrated that ELISA using the LMS antigen yielded more reliable results than IFAT. The LMS antigen displayed no cross-reactivity with sera from patients or dogs that had any of the other diseases tested.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.