ArticleBiophysical journal2002
Mechanics of F-actin characterized with microfabricated cantilevers.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 156 citations in OpenAlex.
- Intermediate Filaments: Structure-Mechanics Crosstalk.Sub-cellular biochemistry · 2026Review
- Membrane-Bound Vimentin Filaments Reorganize and Elongate under Strain.Biomacromolecules · 2023Article
- Hydrodynamic and Polyelectrolyte Properties of Actin Filaments: Theory and Experiments.Polymers · 2022Article
- Protein friction and filament bending facilitate contraction of disordered actomyosin networks.Biophysical journal · 2021Article
- Brownian lithographic polymers of steric lock-and-key colloidal linkages.Science advances · 2021Article
- Altered mechanical properties of actin fibers due to breast cancer invasion: parameter identification based on micropipette aspiration and multiscale tensegrity modeling.Medical & biological engineering & computing · 2021Article
- The load dependence and the force-velocity relation in intact myosin filaments from skeletal and smooth muscles.American journal of physiology. Cell physiology · 2020Article
- Cryo-EM Structure of Actin Filaments fromThe Plant cell · 2019Article
- Response of an actin network in vesicles under electric pulses.Scientific reports · 2019Article
- Response of biopolymer networks governed by the physical properties of cross-linking molecules.Soft matter · 2016Article
- A biomechanical perspective on stress fiber structure and function.Biochimica et biophysica acta · 2015Review
- Mechanics and dynamics of reconstituted cytoskeletal systems.Biochimica et biophysica acta · 2015Review
- Two fundamental mechanisms govern the stiffening of cross-linked networks.Biophysical journal · 2015Article
- Tensegrity, cellular biophysics, and the mechanics of living systems.Reports on progress in physics. Physical Society (Great Britain) · 2014Review
- Structure and mechanical characterization of DNA i-motif nanowires by molecular dynamics simulation.Biophysical journal · 2013Article
- The effects of Ca2+ and MgADP on force development during and after muscle length changes.PloS one · 2013Article
- Physical properties of polymorphic yeast prion amyloid fibers.Biophysical journal · 2011Article
- The effect of muscle length on force depression after active shortening in soleus muscle of mice.European journal of applied physiology · 2011Article
- Stress transmission within the cell.Comprehensive Physiology · 2011Review
- Comparative biomechanics of thick filaments and thin filaments with functional consequences for muscle contraction.Journal of biomedicine & biotechnology · 2010Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this report we characterized the longitudinal elasticity of single actin filaments manipulated by novel silicon-nitride microfabricated levers. Single actin filaments were stretched from zero tension to maximal physiological tension, P(0). The obtained length-tension relation was nonlinear in the low-tension range (0-50 pN) with a resultant strain of approximately 0.4-0.6% and then became linear at moderate to high tensions (approximately 50-230 pN). In this region, the stretching stiffness of a single rhodamine-phalloidin-labeled, 1-microm-long F-actin is 34.5 +/- 3.5 pN/nm. Such a length-tension relation could be characterized by an entropic-enthalpic worm-like chain model, which ascribes most of the energy consumed in the nonlinear portion to overcoming thermal undulations arising from the filament's interaction with surrounding solution and the linear portion to the intrinsic stretching elasticity. By fitting the experimental data with such a worm-like chain model, an estimation of persistence length of approximately 8.75 microm was derived. These results suggest that F-actin is more compliant than previously thought and that thin filament compliance may account for a substantial fraction of the sarcomere's elasticity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.