ArticleBiophysical journal2002
Ca(2+)-dependent interaction between FKBP12 and calcineurin regulates activity of the Ca(2+) release channel in skeletal muscle.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Motor Unit Force Potentiation and Calcium Handling Protein Concentration in Rat Fast Muscle After Resistance Training.Frontiers in physiology · 2021Article
- FK506 regulates CaBritish journal of pharmacology · 2020Article
- FKBP12 contributes to α-synuclein toxicity by regulating the calcineurin-dependent phosphoproteome.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The structural basis of ryanodine receptor ion channel function.The Journal of general physiology · 2017Review
- Raptor ablation in skeletal muscle decreases Cav1.1 expression and affects the function of the excitation-contraction coupling supramolecular complex.The Biochemical journal · 2015Article
- FK506-Binding Proteins and Their Diverse Functions.Current molecular pharmacology · 2015Review
- Calcineurin upregulates local Ca(2+) signaling through ryanodine receptor-1 in airway smooth muscle cells.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Combining fragment homology modeling with molecular dynamics aims at prediction of Ca²⁺ binding sites in CaBPs.Journal of computer-aided molecular design · 2013Article
- Comparative analysis of different peptidyl-prolyl isomerases reveals FK506-binding protein 12 as the most potent enhancer of alpha-synuclein aggregation.The Journal of biological chemistry · 2011Article
- Article
- Regulation of Ryanodine Receptor Ion Channels Through Posttranslational Modifications.Current topics in membranes · 2010Article
- Homer and the ryanodine receptor.European biophysics journal : EBJ · 2009Review
- Regulation by FK506 and rapamycin of Ca2+ release from the sarcoplasmic reticulum in vascular smooth muscle: the role of FK506 binding proteins and mTOR.British journal of pharmacology · 2009Article
- Constitutive expression of suppressor of cytokine signalling-3 in skeletal muscle leads to reduced mobility and overweight in mice.Diabetologia · 2009Article
- Three-dimensional visualization of FKBP12.6 binding to an open conformation of cardiac ryanodine receptor.Biophysical journal · 2006Article
- Regulation of the voltage-gated potassium channel KCNQ4 in the auditory pathway.Pflugers Archiv : European journal of physiology · 2005Article
- Selective inhibition of calcineurin-NFAT signaling by blocking protein-protein interaction with small organic molecules.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Calmodulin modulates initiation but not termination of spontaneous Ca2+ sparks in frog skeletal muscle.Biophysical journal · 2003Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Calcineurin is a Ca(2+) and calmodulin-dependent protein phosphatase with diverse cellular functions. Here we examined the physical and functional interactions between calcineurin and ryanodine receptor (RyR) in a C2C12 cell line derived from mouse skeletal muscle. Coimmunoprecipitation experiments revealed that the association between RyR and calcineurin exhibits a strong Ca(2+) dependence. This association involves a Ca(2+) dependent interaction between calcineurin and FK506-binding protein (FKBP12), an accessory subunit of RyR. Pretreatment with cyclosporin A, an inhibitor of calcineurin, enhanced the caffeine-induced Ca(2+) release (CICR) in C2C12 cells. This effect was similar to those of FK506 and rapamycin, two drugs known to cause dissociation of FKBP12 from RyR. Overexpression of a constitutively active form of calcineurin in C2C12 cells, DeltaCnA(391-521) (deletion of the last 131 amino acids from calcineurin), resulted in a decrease in CICR. This decrease in CICR activity was partially recovered by pretreatment with cyclosporin A. Furthermore, overexpression of an endogenous calcineurin inhibitor (cain) or an inactive form of calcineurin (DeltaCnA(H101Q)) in C2C12 cells resulted in up-regulation of CICR. Taken together, our data suggest that a trimeric-interaction among calcineurin, FKBP12, and RyR is important for the regulation of the RyR channel activity and may play an important role in the Ca(2+) signaling of muscle contraction and relaxation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.