ArticleBiophysical journal2002
SNAREs in opposing bilayers interact in a circular array to form conducting pores.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed, 111 citations in OpenAlex.
- Article
- Nanoscale imaging using differential expansion microscopy.Histochemistry and cell biology · 2020Article
- Neuronal Porosome Complex: Secretory Machinery at the Nerve Terminal.Discoveries (Craiova, Romania) · 2017Review
- Human Platelet Vesicles Exhibit Distinct Size and Proteome.Journal of proteome research · 2017Article
- Fusion Pore: A Curious Case of Rediscovering Science.Discoveries (Craiova, Romania) · 2017Article
- Function Suggests Nano-Structure: Quantitative Structural Support for SNARE-Mediated Pore Formation.Neurotoxicity research · 2016Article
- The neuronal porosome complex in health and disease.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Membrane Repair: Mechanisms and Pathophysiology.Physiological reviews · 2015Review
- 'Porosome' discovered nearly 20 years ago provides molecular insights into the kiss-and-run mechanism of cell secretion.Journal of cellular and molecular medicine · 2015Review
- Mutant SNAP25B causes myasthenia, cortical hyperexcitability, ataxia, and intellectual disability.Neurology · 2014Article
- Porosome: a membrane microdomain acting as the universal secretory portal in exocytosis.Discoveries (Craiova, Romania) · 2014Review
- Insights into the Nanobiology of Growth Hormone Secretion.Discoveries (Craiova, Romania) · 2014Article
- NeuronalJournal of molecular structure · 2014Article
- Lysophosphatidylcholine inhibits membrane-associated SNARE complex disassembly.Journal of cellular and molecular medicine · 2012Article
- Neuronal porosome proteome: Molecular dynamics and architecture.Journal of proteomics · 2012Article
- 3D organization and function of the cell: Golgi budding and vesicle biogenesis to docking at the porosome complex.Histochemistry and cell biology · 2012Article
- Membrane-directed molecular assembly of the neuronal SNARE complex.Journal of cellular and molecular medicine · 2011Article
- Membrane lipids influence protein complex assembly-disassembly.Journal of the American Chemical Society · 2010Article
- Structure of membrane-associated neuronal SNARE complex: implication in neurotransmitter release.Journal of cellular and molecular medicine · 2009Article
- Investigation of SNARE-Mediated Membrane Fusion Mechanism Using Atomic Force Microscopy.Japanese journal of applied physics (2008) · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The process of fusion at the nerve terminal is mediated via a specialized set of proteins in the synaptic vesicles and the presynaptic membrane. Three soluble N-ethylmaleimide-sensitive factor (NSF)-attachment protein receptors (SNAREs) have been implicated in membrane fusion. The structure and arrangement of these SNAREs associated with lipid bilayers were examined using atomic force microscopy. A bilayer electrophysiological setup allowed for measurements of membrane conductance and capacitance. Here we demonstrate that the interaction of these proteins to form a fusion pore is dependent on the presence of t-SNAREs and v-SNARE in opposing bilayers. Addition of purified recombinant v-SNARE to a t-SNARE-reconstituted lipid membrane increased only the size of the globular t-SNARE oligomer without influencing the electrical properties of the membrane. However when t-SNARE vesicles were added to a v-SNARE membrane, SNAREs assembles in a ring pattern and a stepwise increase in capacitance, and increase in conductance were observed. Thus, t- and v-SNAREs are required to reside in opposing bilayers to allow appropriate t-/v-SNARE interactions leading to membrane fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.