ArticleBiophysical journal2002
Hyperosmotically induced volume change and calcium signaling in intervertebral disk cells: the role of the actin cytoskeleton.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Modulation of TRPV4 protects against degeneration induced by sustained loading and promotes matrix synthesis in the intervertebral disc.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- From Mechanobiology to Mechanical Repair Strategies: A Bibliometric Analysis of Biomechanical Studies of Intervertebral Discs.Journal of pain research · 2022Review
- Compressive mechanical stress enhances susceptibility to interleukin-1 by increasing interleukin-1 receptor expression in 3D-cultured ATDC5 cells.BMC musculoskeletal disorders · 2021Article
- Identification and Characterization of Serum microRNAs as Biomarkers for Human Disc Degeneration: An RNA Sequencing Analysis.Diagnostics (Basel, Switzerland) · 2020Article
- Actomyosin contractility confers mechanoprotection against TNFα-induced disruption of the intervertebral disc.Science advances · 2020Article
- Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration.Clinical orthopaedics and related research · 2019Article
- Expression and Activity of TRPA1 and TRPV1 in the Intervertebral Disc: Association with Inflammation and Matrix Remodeling.International journal of molecular sciences · 2019Article
- Notochordal cell matrix: An inhibitor of neurite and blood vessel growth?Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- p38 MAPK Facilitates Crosstalk Between Endoplasmic Reticulum Stress and IL-6 Release in the Intervertebral Disc.Frontiers in immunology · 2018Article
- Identification of key genes associated with the effect of osmotic stimuli on intervertebral discs using microarray analysis.Oncology letters · 2017Article
- Article
- Reduced tissue osmolarity increases TRPV4 expression and pro-inflammatory cytokines in intervertebral disc cells.European cells & materials · 2016Article
- Impact of cellular microenvironment and mechanical perturbation on calcium signalling in meniscus fibrochondrocytes.European cells & materials · 2014Article
- Inflammation induces irreversible biophysical changes in isolated nucleus pulposus cells.PloS one · 2014Article
- Gene expression analysis in response to osmotic stimuli in the intervertebral disc with DNA microarray.European journal of medical research · 2013Article
- Tonicity-independent regulation of the osmosensitive transcription factor TonEBP (NFAT5).American journal of physiology. Cell physiology · 2012Review
- Osmotic stress alters chromatin condensation and nucleocytoplasmic transport.Biochemical and biophysical research communications · 2011Article
- Influence of the partitioning of osmolytes by the cytoplasm on the passive response of cells to osmotic loading.Biophysical journal · 2009Article
- Osmolarity and intracellular calcium regulate aquaporin2 expression through TonEBP in nucleus pulposus cells of the intervertebral disc.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2009Article
- Retinal insulin receptor signaling in hyperosmotic stress.Vitamins and hormones · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Loading of the spine alters the osmotic environment in the intervertebral disk (IVD) as interstitial water is expressed from the tissue. Cells from the three zones of the IVD, the anulus fibrosus (AF), transition zone (TZ), and nucleus pulposus (NP), respond to osmotic stress with altered biosynthesis through a pathway that may involve calcium (Ca(2+)) as a second messenger. We examined the hypothesis that IVD cells respond to hyperosmotic stress by increasing the concentration of intracellular calcium ([Ca(2+)](i)) through a mechanism involving F-actin. In response to hyperosmotic stress, control cells from all zones decreased in volume and cells from the AF and TZ exhibited [Ca(2+)](i) transients, while cells from the NP did not. Extracellular Ca(2+) was necessary to initiate [Ca(2+)](i) transients. Stabilization of F-actin with phalloidin prevented the Ca(2+) response in AF and TZ cells and decreased the rate of volume change in cells from all zones, coupled with an increase in the elastic moduli and apparent viscosity. Conversely, actin breakdown with cytochalasin D facilitated Ca(2+) signaling while decreasing the elastic moduli and apparent viscosity for NP cells. These results suggest that hyperosmotic stress induces volume change in IVD cells and may initiate [Ca(2+)](i) transients through an actin-dependent mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.